ALG5 Gene

HGNC Family	Glycosyltransferases
Name	ALG5, dolichyl-phosphate beta-glucosyltransferase
Description	This gene encodes a member of the glycosyltransferase 2 family. The encoded protein participates in glucosylation of the oligomannose core in N-linked glycosylation of proteins. The addition of glucose residues to the oligomannose core is necessary to ensure substrate recognition, and therefore, effectual transfer of the oligomannose core to the nascent glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n ALG5 encodes a dolichol phosphate glucosyltransferase that catalyzes the synthesis of dolichol‐phosphate–glucose from UDP‐glucose and dolichol phosphate. This lipid‐linked glucose donor is essential for the proper assembly of the oligosaccharide precursors required for N‐linked glycosylation in the endoplasmic reticulum. Studies in budding yeast and in the protist Trichomonas vaginalis have demonstrated that ALG5 activity is necessary for efficient protein glycosylation, as its disruption leads to an accumulation of underglucosylated lipid‐linked oligosaccharides and consequent defects in glycoprotein maturation [(1,2,3,4,5,6,7,8)]. In yeast, for example, alg5 mutants show reduced incorporation of glucose into the growing precursor, which in turn diminishes the efficiency with which the glycan is transferred to nascent proteins [(2,3,4,5,6,7,8)]. Likewise, recombinant expression of Trichomonas ALG5 proteins has been shown to restore normal glycosylation in a yeast alg5 mutant, confirming the conserved function of this enzyme in dolichol phosphate–glucose synthesis and its role in the proper glucosylation of both N‐ and, in some cases, O‐linked glycans [(1)]. In addition, human genetic studies have functionally complemented yeast alg5 deficiencies with ALG5 cDNA, underscoring the enzyme’s critical role in glycoprotein biosynthesis and its potential involvement in congenital disorders of glycosylation when mutant or deficient [(9)]. Overall, the conserved enzymatic activity of ALG5 is central to maintaining the fidelity of glycosylation processes in eukaryotes."}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "fg", "children": [{"type": "fg_fs", "start_ref": "1", "end_ref": "9"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Kariona A Grabińska, Sudip K Ghosh, Ziqiang Guan, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Dolichyl-phosphate-glucose is used to make O-glycans on glycoproteins of Trichomonas vaginalis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Eukaryot Cell (2008)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1128/EC.00061-08"}], "href": "https://doi.org/10.1128/EC.00061-08"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "18552282"}], "href": "https://pubmed.ncbi.nlm.nih.gov/18552282"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "L Ballou, P Gopal, B Krummel, et al. "}, {"type": "b", "children": [{"type": "t", "text": "A mutation that prevents glucosylation of the lipid-linked oligosaccharide precursor leads to underglycosylation of secreted yeast invertase."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Proc Natl Acad Sci U S A (1986)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1073/pnas.83.10.3081"}], "href": "https://doi.org/10.1073/pnas.83.10.3081"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "3517849"}], "href": "https://pubmed.ncbi.nlm.nih.gov/3517849"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "T C Huffaker, P W Robbins "}, {"type": "b", "children": [{"type": "t", "text": "Yeast mutants deficient in protein glycosylation."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Proc Natl Acad Sci U S A (1983)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1073/pnas.80.24.7466"}], "href": "https://doi.org/10.1073/pnas.80.24.7466"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "6369318"}], "href": "https://pubmed.ncbi.nlm.nih.gov/6369318"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "K W Runge, T C Huffaker, P W Robbins "}, {"type": "b", "children": [{"type": "t", "text": "Two yeast mutations in glucosylation steps of the asparagine glycosylation pathway."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (1984)"}]}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "6423630"}], "href": "https://pubmed.ncbi.nlm.nih.gov/6423630"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "M D Muñoz, L M Hernández, R Basco, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Glycosylation of yeast exoglucanase sequons in alg mutants deficient in the glucosylation steps of the lipid-linked oligosaccharide. Presence of glucotriose unit in Dol-PP-GlcNAc2Man9Glc3 influences both glycosylation efficiency and selection of N-linked sites."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biochim Biophys Acta (1994)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/0304-4165(94)90063-9"}], "href": "https://doi.org/10.1016/0304-4165(94"}, {"type": "t", "text": "90063-9) PMID: "}, {"type": "a", "children": [{"type": "t", "text": "7803465"}], "href": "https://pubmed.ncbi.nlm.nih.gov/7803465"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "S Heesen, L Lehle, A Weissmann, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Isolation of the ALG5 locus encoding the UDP-glucose:dolichyl-phosphate glucosyltransferase from Saccharomyces cerevisiae."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Eur J Biochem (1994)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1111/j.1432-1033.1994.tb19996.x"}], "href": "https://doi.org/10.1111/j.1432-1033.1994.tb19996.x"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "8076653"}], "href": "https://pubmed.ncbi.nlm.nih.gov/8076653"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "I Stagljar, C Korostensky, N Johnsson, et al. "}, {"type": "b", "children": [{"type": "t", "text": "A genetic system based on split-ubiquitin for the analysis of interactions between membrane proteins in vivo."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Proc Natl Acad Sci U S A (1998)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1073/pnas.95.9.5187"}], "href": "https://doi.org/10.1073/pnas.95.9.5187"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "9560251"}], "href": "https://pubmed.ncbi.nlm.nih.gov/9560251"}]}, {"type": "r", "ref": 8, "children": [{"type": "t", "text": "O Castro, L Y Chen, A J Parodi, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Uridine diphosphate-glucose transport into the endoplasmic reticulum of Saccharomyces cerevisiae: in vivo and in vitro evidence."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Biol Cell (1999)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1091/mbc.10.4.1019"}], "href": "https://doi.org/10.1091/mbc.10.4.1019"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "10198054"}], "href": "https://pubmed.ncbi.nlm.nih.gov/10198054"}]}, {"type": "r", "ref": 9, "children": [{"type": "t", "text": "T Imbach, P Burda, P Kuhnert, et al. "}, {"type": "b", "children": [{"type": "t", "text": "A mutation in the human ortholog of the Saccharomyces cerevisiae ALG6 gene causes carbohydrate-deficient glycoprotein syndrome type-Ic."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Proc Natl Acad Sci U S A (1999)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1073/pnas.96.12.6982"}], "href": "https://doi.org/10.1073/pnas.96.12.6982"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "10359825"}], "href": "https://pubmed.ncbi.nlm.nih.gov/10359825"}]}]}]}
Synonyms	BA421P11.2
Proteins	ALG5_HUMAN
NCBI Gene ID	29880
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Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

ALG5 has 5,878 functional associations with biological entities spanning 8 categories (molecular profile, organism, chemical, functional term, phrase or reference, disease, phenotype or trait, structural feature, cell line, cell type or tissue, gene, protein or microRNA) extracted from 117 datasets.

Click the + buttons to view associations for ALG5 from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

ALG5 Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Achilles Cell Line Gene Essentiality Profiles	cell lines with fitness changed by ALG5 gene knockdown relative to other cell lines from the Achilles Cell Line Gene Essentiality Profiles dataset.
	Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of ALG5 gene relative to other tissues from the Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles	tissues with high or low expression of ALG5 gene relative to other tissues from the Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles	tissue samples with high or low expression of ALG5 gene relative to other tissue samples from the Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of ALG5 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq	tissue samples with high or low expression of ALG5 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of ALG5 gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	BioGPS Cell Line Gene Expression Profiles	cell lines with high or low expression of ALG5 gene relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset.
	BioGPS Human Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of ALG5 gene relative to other cell types and tissues from the BioGPS Human Cell Type and Tissue Gene Expression Profiles dataset.
	BioGPS Mouse Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of ALG5 gene relative to other cell types and tissues from the BioGPS Mouse Cell Type and Tissue Gene Expression Profiles dataset.
	Carcinogenome Chemical Perturbation Carcinogenicity Signatures	small molecule perturbations changing expression of ALG5 gene from the Carcinogenome Chemical Perturbation Carcinogenicity Signatures dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of ALG5 gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Gene Expression Profiles	cell lines with high or low expression of ALG5 gene relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.
	CCLE Cell Line Proteomics	Cell lines associated with ALG5 protein from the CCLE Cell Line Proteomics dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of ALG5 gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of ALG5 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of ALG5 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	CMAP Signatures of Differentially Expressed Genes for Small Molecules	small molecule perturbations changing expression of ALG5 gene from the CMAP Signatures of Differentially Expressed Genes for Small Molecules dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores	cellular components containing ALG5 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores 2025	cellular components containing ALG5 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores	cellular components containing ALG5 protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores 2025	cellular components containing ALG5 protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores	cellular components co-occuring with ALG5 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with ALG5 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	COSMIC Cell Line Gene CNV Profiles	cell lines with high or low copy number of ALG5 gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with ALG5 gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Chemical Interactions	chemicals interacting with ALG5 gene/protein from the curated CTD Gene-Chemical Interactions dataset.
	CTD Gene-Disease Associations	diseases associated with ALG5 gene/protein from the curated CTD Gene-Disease Associations dataset.
	DeepCoverMOA Drug Mechanisms of Action	small molecule perturbations with high or low expression of ALG5 protein relative to other small molecule perturbations from the DeepCoverMOA Drug Mechanisms of Action dataset.
	DepMap CRISPR Gene Dependency	cell lines with fitness changed by ALG5 gene knockdown relative to other cell lines from the DepMap CRISPR Gene Dependency dataset.
	DISEASES Experimental Gene-Disease Association Evidence Scores 2025	diseases associated with ALG5 gene in GWAS datasets from the DISEASES Experimental Gene-Disease Assocation Evidence Scores 2025 dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with ALG5 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores 2025	diseases co-occuring with ALG5 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores 2025 dataset.
	DisGeNET Gene-Phenotype Associations	phenotypes associated with ALG5 gene in GWAS and other genetic association datasets from the DisGeNET Gene-Phenoptype Associations dataset.
	ENCODE Histone Modification Site Profiles	histone modification site profiles with high histone modification abundance at ALG5 gene from the ENCODE Histone Modification Site Profiles dataset.
	ENCODE Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of ALG5 gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
	ENCODE Transcription Factor Targets	transcription factors binding the promoter of ALG5 gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.
	ESCAPE Omics Signatures of Genes and Proteins for Stem Cells	PubMedIDs of publications reporting gene signatures containing ALG5 from the ESCAPE Omics Signatures of Genes and Proteins for Stem Cells dataset.
	GDSC Cell Line Gene Expression Profiles	cell lines with high or low expression of ALG5 gene relative to other cell lines from the GDSC Cell Line Gene Expression Profiles dataset.
	GeneSigDB Published Gene Signatures	PubMedIDs of publications reporting gene signatures containing ALG5 from the GeneSigDB Published Gene Signatures dataset.