{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n ANO1‐AS2 is a long non‐coding RNA that appears to play a regulatory role by acting on its neighboring ANO1 gene. In testicular tissue, ANO1‐AS2 is significantly upregulated in patients with asthenozoospermia and terato‐asthenozoospermia, where its increased expression is inversely correlated with ANO1 expression. Functional analyses indicate that ANO1‐AS2 can bind to the promoter region of the ANO1 gene, thereby downregulating its transcription. Given that ANO1 expression is positively associated with normal sperm motility and morphology, the upregulation of ANO1‐AS2 is likely to contribute to impaired sperm function and male infertility."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n Moreover, ANO1‐AS2 has been identified as one of several significantly upregulated lncRNAs in acute lymphoblastic leukemia (ALL) bone marrow samples. Although its specific mechanistic role in ALL remains to be fully defined, its inclusion in a competing endogenous RNA network suggests that ANO1‐AS2 may be involved in modulating gene expression patterns that contribute to leukemogenesis."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n Thus, across distinct pathological contexts, ANO1‐AS2 appears to function as a negative regulator of ANO1 gene expression in the reproductive system, with potential implications in sperm motility and morphology, and may also participate in broader regulatory networks in cancer. \n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Mohammadreza Saberiyan, Reza Mirfakhraie, Delnya Gholami, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Investigating the regulatory function of the ANO1-AS2 on the ANO1 gene in infertile men with asthenozoospermia and terato-asthenozoospermia."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Exp Mol Pathol (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.yexmp.2020.104528"}], "href": "https://doi.org/10.1016/j.yexmp.2020.104528"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "32916161"}], "href": "https://pubmed.ncbi.nlm.nih.gov/32916161"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Weimin Wang, Chunhui Lyu, Fei Wang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Identification of Potential Signatures and Their Functions for Acute Lymphoblastic Leukemia: A Study Based on the Cancer Genome Atlas."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Front Genet (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3389/fgene.2021.656042"}], "href": "https://doi.org/10.3389/fgene.2021.656042"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34295352"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34295352"}]}]}]}