ANP32E Gene

HGNC Family	ANP32 acidic nuclear phosphoproteins (ANP32)
Name	acidic (leucine-rich) nuclear phosphoprotein 32 family, member E
Description	Enables histone binding activity; histone chaperone activity; and protein folding chaperone. Predicted to be involved in regulation of apoptotic process. Located in nucleus. Part of Swr1 complex. [provided by Alliance of Genome Resources, Mar 2025]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n ANP32E is an acidic nuclear phosphoprotein that functions as a highly specific histone chaperone for the H2A.Z variant. Structural and biochemical studies reveal that ANP32E directly engages the H2A.Z–H2B dimer—via a dedicated interacting domain—to evict H2A.Z from nucleosomes. This activity refines nucleosome positioning and regulates chromatin accessibility, thereby modulating transcriptional activity and ensuring proper gene expression. In the context of DNA damage, ANP32E is rapidly recruited to sites of double‐strand breaks where, in collaboration with ATP‐dependent remodelers such as INO80, its H2A.Z removal facilitates homologous recombination and efficient repair ("}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "1", "end_ref": "4"}]}, {"type": "t", "text": ").\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n Beyond its role in chromatin remodeling, ANP32E is increasingly recognized as an important modulator in oncogenesis. In several cancers—including breast, thyroid, pancreatic, and lung adenocarcinoma—elevated ANP32E levels promote cell proliferation, migration, and invasion. This pro-tumorigenic impact is mediated, in part, by transcriptional activation of cell cycle regulators (for example, through E2F1 induction and β-catenin/cyclin D1 signaling) and by modulation of metabolic pathways such as AKT/mTOR-driven glycolysis. Moreover, deregulation of ANP32E via suppression by microRNAs (e.g. miR-141 and let-7c) further underscores its role in driving tumor aggressiveness ("}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "5", "end_ref": "9"}]}, {"type": "t", "text": ").\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n In addition, ANP32E plays a crucial role in non-dividing cells such as neurons by maintaining basal chromatin states. Its function in preventing excessive accumulation of H2A.Z is essential for proper transcriptional regulation, dendritic arborization, and memory formation. Furthermore, ANP32E appears to protect H2A.Z from proteasomal degradation via mechanisms involving phosphorylation, thereby linking its chaperone activity to broader chromatin signaling networks ("}, {"type": "fg", "children": [{"type": "fg_f", "ref": "10"}]}, {"type": "t", "text": ").\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n Finally, alterations in ANP32E expression have been implicated in cancer progression and aggressiveness including roles as a potential driver in multiple myeloma. Its expression levels have also been correlated with chromatin state changes that influence cellular differentiation and tumor plasticity ("}, {"type": "fg", "children": [{"type": "fg_f", "ref": "12"}]}, {"type": "t", "text": ").\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n Collectively, these findings position ANP32E as a critical regulator of chromatin dynamics, whose histone chaperone activity for H2A.Z plays essential roles in transcription, DNA repair, and cell fate decisions, while its dysregulation contributes to oncogenic transformation and tumor progression.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Arnaud Obri, Khalid Ouararhni, Christophe Papin, et al. "}, {"type": "b", "children": [{"type": "t", "text": "ANP32E is a histone chaperone that removes H2A.Z from chromatin."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nature (2014)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/nature12922"}], "href": "https://doi.org/10.1038/nature12922"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "24463511"}], "href": "https://pubmed.ncbi.nlm.nih.gov/24463511"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Hanan E Alatwi, Jessica A Downs "}, {"type": "b", "children": [{"type": "t", "text": "Removal of H2A.Z by INO80 promotes homologous recombination."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "EMBO Rep (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.15252/embr.201540330"}], "href": "https://doi.org/10.15252/embr.201540330"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26142279"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26142279"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Ozge Gursoy-Yuzugullu, Marina K Ayrapetov, Brendan D Price "}, {"type": "b", "children": [{"type": "t", "text": "Histone chaperone Anp32e removes H2A.Z from DNA double-strand breaks and promotes nucleosome reorganization and DNA repair."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Proc Natl Acad Sci U S A (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1073/pnas.1504868112"}], "href": "https://doi.org/10.1073/pnas.1504868112"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26034280"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26034280"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Zhuo Mao, Lu Pan, Weixiang Wang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Anp32e, a higher eukaryotic histone chaperone directs preferential recognition for H2A.Z."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell Res (2014)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/cr.2014.30"}], "href": "https://doi.org/10.1038/cr.2014.30"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "24613878"}], "href": "https://pubmed.ncbi.nlm.nih.gov/24613878"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Ping Li, Tao Xu, Xin Zhou, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Downregulation of miRNA-141 in breast cancer cells is associated with cell migration and invasion: involvement of ANP32E targeting."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cancer Med (2017)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1002/cam4.1024"}], "href": "https://doi.org/10.1002/cam4.1024"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "28220627"}], "href": "https://pubmed.ncbi.nlm.nih.gov/28220627"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Zhenchong Xiong, Liping Ye, He Zhenyu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "ANP32E induces tumorigenesis of triple-negative breast cancer cells by upregulating E2F1."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Oncol (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1002/1878-0261.12202"}], "href": "https://doi.org/10.1002/1878-0261.12202"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "29633513"}], "href": "https://pubmed.ncbi.nlm.nih.gov/29633513"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Junwei Huang, Wen Gao, Hongfei Liu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Up-regulated ANP32E promotes the thyroid carcinoma cell proliferation and migration via activating AKT/mTOR/HK2-mediated glycolysis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Gene (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.gene.2020.144681"}], "href": "https://doi.org/10.1016/j.gene.2020.144681"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "32304784"}], "href": "https://pubmed.ncbi.nlm.nih.gov/32304784"}]}, {"type": "r", "ref": 8, "children": [{"type": "t", "text": "Jianwei Zhang, Zhongmin Lan, Guotong Qiu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Over-expression of ANP32E is associated with poor prognosis of pancreatic cancer and promotes cell proliferation and migration through regulating β-catenin."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "BMC Cancer (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1186/s12885-020-07556-z"}], "href": "https://doi.org/10.1186/s12885-020-07556-z"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "33148205"}], "href": "https://pubmed.ncbi.nlm.nih.gov/33148205"}]}, {"type": "r", "ref": 9, "children": [{"type": "t", "text": "Lei Wang, Jun Li, Yan Li, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Hsa-let-7c exerts an anti-tumor function by negatively regulating ANP32E in lung adenocarcinoma."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Tissue Cell (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.tice.2020.101372"}], "href": "https://doi.org/10.1016/j.tice.2020.101372"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "32746998"}], "href": "https://pubmed.ncbi.nlm.nih.gov/32746998"}]}, {"type": "r", "ref": 10, "children": [{"type": "t", "text": "Hyewon Shin, Minzhen He, Zhi Yang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Transcriptional regulation mediated by H2A.Z via ANP32e-dependent inhibition of protein phosphatase 2A."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biochim Biophys Acta Gene Regul Mech (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.bbagrm.2018.03.002"}], "href": "https://doi.org/10.1016/j.bbagrm.2018.03.002"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "29524612"}], "href": "https://pubmed.ncbi.nlm.nih.gov/29524612"}]}, {"type": "r", "ref": 11, "children": [{"type": "t", "text": "Gilda Stefanelli, Claire E Makowski, Mark A Brimble, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The histone chaperone Anp32e regulates memory formation, transcription, and dendritic morphology by regulating steady-state H2A.Z binding in neurons."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell Rep (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.celrep.2021.109551"}], "href": "https://doi.org/10.1016/j.celrep.2021.109551"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34407406"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34407406"}]}, {"type": "r", "ref": 12, "children": [{"type": "t", "text": "Garrett L Ruff, Kristin E Murphy, Zachary R Smith, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Subtype-Independent ANP32E Reduction During Breast Cancer Progression in Accordance with Chromatin Relaxation."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "BMC Cancer (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1186/s12885-021-09077-9"}], "href": "https://doi.org/10.1186/s12885-021-09077-9"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34922480"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34922480"}]}, {"type": "r", "ref": 13, "children": [{"type": "t", "text": "Can Li, Erik B Wendlandt, Benjamin Darbro, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Genetic Analysis of Multiple Myeloma Identifies Cytogenetic Alterations Implicated in Disease Complexity and Progression."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cancers (Basel) (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3390/cancers13030517"}], "href": "https://doi.org/10.3390/cancers13030517"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "33572851"}], "href": "https://pubmed.ncbi.nlm.nih.gov/33572851"}]}]}]}
Synonyms	LANP-L, LANPL
Proteins	AN32E_HUMAN
NCBI Gene ID	81611
API
Download Associations
Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

ANP32E has 9,354 functional associations with biological entities spanning 8 categories (molecular profile, organism, chemical, functional term, phrase or reference, disease, phenotype or trait, structural feature, cell line, cell type or tissue, gene, protein or microRNA) extracted from 115 datasets.

Click the + buttons to view associations for ANP32E from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

ANP32E Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Achilles Cell Line Gene Essentiality Profiles	cell lines with fitness changed by ANP32E gene knockdown relative to other cell lines from the Achilles Cell Line Gene Essentiality Profiles dataset.
	Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of ANP32E gene relative to other tissues from the Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles	tissue samples with high or low expression of ANP32E gene relative to other tissue samples from the Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of ANP32E gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq	tissue samples with high or low expression of ANP32E gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of ANP32E gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	BioGPS Cell Line Gene Expression Profiles	cell lines with high or low expression of ANP32E gene relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset.
	BioGPS Human Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of ANP32E gene relative to other cell types and tissues from the BioGPS Human Cell Type and Tissue Gene Expression Profiles dataset.
	BioGPS Mouse Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of ANP32E gene relative to other cell types and tissues from the BioGPS Mouse Cell Type and Tissue Gene Expression Profiles dataset.
	Carcinogenome Chemical Perturbation Carcinogenicity Signatures	small molecule perturbations changing expression of ANP32E gene from the Carcinogenome Chemical Perturbation Carcinogenicity Signatures dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of ANP32E gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Gene Expression Profiles	cell lines with high or low expression of ANP32E gene relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.
	CCLE Cell Line Proteomics	Cell lines associated with ANP32E protein from the CCLE Cell Line Proteomics dataset.
	CellMarker Gene-Cell Type Associations	cell types associated with ANP32E gene from the CellMarker Gene-Cell Type Associations dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of ANP32E gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of ANP32E gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of ANP32E gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	CM4AI KOLF21J CRISPRi Gene Perturbation Atlas	gene perturbations changing expression of ANP32E gene from the CM4AI KOLF21J CRISPRi Gene Perturbation Atlas dataset.
	CM4AI U2OS Cell Map Protein Localization Assemblies	assemblies containing ANP32E protein from integrated AP-MS and IF data from the CM4AI U2OS Cell Map Protein Localization Assemblies dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores	cellular components containing ANP32E protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores 2025	cellular components containing ANP32E protein from the COMPARTMENTS Curated Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores	cellular components co-occuring with ANP32E protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with ANP32E protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	COSMIC Cell Line Gene CNV Profiles	cell lines with high or low copy number of ANP32E gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with ANP32E gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Chemical Interactions	chemicals interacting with ANP32E gene/protein from the curated CTD Gene-Chemical Interactions dataset.
	CTD Gene-Disease Associations	diseases associated with ANP32E gene/protein from the curated CTD Gene-Disease Associations dataset.
	DeepCoverMOA Drug Mechanisms of Action	small molecule perturbations with high or low expression of ANP32E protein relative to other small molecule perturbations from the DeepCoverMOA Drug Mechanisms of Action dataset.
	DepMap CRISPR Gene Dependency	cell lines with fitness changed by ANP32E gene knockdown relative to other cell lines from the DepMap CRISPR Gene Dependency dataset.
	DISEASES Experimental Gene-Disease Association Evidence Scores 2025	diseases associated with ANP32E gene in GWAS datasets from the DISEASES Experimental Gene-Disease Assocation Evidence Scores 2025 dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with ANP32E gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores 2025	diseases co-occuring with ANP32E gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores 2025 dataset.
	DisGeNET Gene-Disease Associations	diseases associated with ANP32E gene in GWAS and other genetic association datasets from the DisGeNET Gene-Disease Associations dataset.
	DisGeNET Gene-Phenotype Associations	phenotypes associated with ANP32E gene in GWAS and other genetic association datasets from the DisGeNET Gene-Phenoptype Associations dataset.
	ENCODE Histone Modification Site Profiles	histone modification site profiles with high histone modification abundance at ANP32E gene from the ENCODE Histone Modification Site Profiles dataset.
	ENCODE Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of ANP32E gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
	ENCODE Transcription Factor Targets	transcription factors binding the promoter of ANP32E gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.
	ESCAPE Omics Signatures of Genes and Proteins for Stem Cells	PubMedIDs of publications reporting gene signatures containing ANP32E from the ESCAPE Omics Signatures of Genes and Proteins for Stem Cells dataset.
	GDSC Cell Line Gene Expression Profiles	cell lines with high or low expression of ANP32E gene relative to other cell lines from the GDSC Cell Line Gene Expression Profiles dataset.
	GeneRIF Biological Term Annotations	biological terms co-occuring with ANP32E gene in literature-supported statements describing functions of genes from the GeneRIF Biological Term Annotations dataset.