APIP Gene

Name	APAF1 interacting protein
Description	Enables identical protein binding activity; methylthioribulose 1-phosphate dehydratase activity; and zinc ion binding activity. Involved in several processes, including L-methionine salvage from methylthioadenosine; protein homotetramerization; and pyroptotic inflammatory response. Located in cytoplasm. [provided by Alliance of Genome Resources, Mar 2025]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n APIP is a multifunctional protein that plays pivotal roles in controlling cell death and cellular metabolism. In its role as an endogenous regulator of the apoptosome, APIP interacts directly with Apaf‐1 by binding its caspase-recruitment (CARD) domain, thereby competing with caspase-9 and suppressing mitochondrial-dependent apoptosis (see."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "1", "end_ref": "3"}]}, {"type": "t", "text": " In addition to this antiapoptotic function, APIP serves as a 5-methylthioribulose-1-phosphate dehydratase within the methionine salvage pathway, an enzymatic activity that appears essential for its ability to inhibit caspase-1–dependent pyroptosis (see."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": " Importantly, these two functions of APIP are separable; its suppression of apoptosis occurs independently of its dehydratase activity, whereas inhibition of pyroptosis depends on a functional methionine salvage pathway. Furthermore, altered expression of APIP is linked to cancer biology. For instance, decreased APIP levels in non-small cell lung carcinoma may lower the threshold for apoptosome activation, while in gastric cancers APIP can enhance ERBB3-dependent signaling and tumorigenesis through promotion of ERBB3–ERBB2 complex formation leading to sustained ERK1/2 and AKT pathway activation."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "6"}]}, {"type": "t", "text": "\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Dong-Hyung Cho, Yeon-Mi Hong, Ho-June Lee, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Induced inhibition of ischemic/hypoxic injury by APIP, a novel Apaf-1-interacting protein."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2004)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M405747200"}], "href": "https://doi.org/10.1074/jbc.M405747200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "15262985"}], "href": "https://pubmed.ncbi.nlm.nih.gov/15262985"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Wonchull Kang, Jin Kuk Yang "}, {"type": "b", "children": [{"type": "t", "text": "Crystallization and preliminary X-ray crystallographic analysis of human Apaf-1-interacting protein."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Acta Crystallogr Sect F Struct Biol Cryst Commun (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1107/S1744309112042832"}], "href": "https://doi.org/10.1107/S1744309112042832"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23192037"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23192037"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Guodong Cao, Michael Xiao, Fengyan Sun, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Cloning of a novel Apaf-1-interacting protein: a potent suppressor of apoptosis and ischemic neuronal cell death."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Neurosci (2004)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1523/JNEUROSCI.1426-04.2004"}], "href": "https://doi.org/10.1523/JNEUROSCI.1426-04.2004"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "15240811"}], "href": "https://pubmed.ncbi.nlm.nih.gov/15240811"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Dennis C Ko, Eric R Gamazon, Kajal P Shukla, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Functional genetic screen of human diversity reveals that a methionine salvage enzyme regulates inflammatory cell death."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Proc Natl Acad Sci U S A (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1073/pnas.1206701109"}], "href": "https://doi.org/10.1073/pnas.1206701109"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22837397"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22837397"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Wonchull Kang, Se Hoon Hong, Hye Min Lee, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Proc Natl Acad Sci U S A (2014)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1073/pnas.1308768111"}], "href": "https://doi.org/10.1073/pnas.1308768111"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "24367089"}], "href": "https://pubmed.ncbi.nlm.nih.gov/24367089"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Erika Moravcikova, Evzen Krepela, Jan Prochazka, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Down-regulated expression of apoptosis-associated genes APIP and UACA in non-small cell lung carcinoma."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Int J Oncol (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3892/ijo.2012.1397"}], "href": "https://doi.org/10.3892/ijo.2012.1397"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22407486"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22407486"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Se-Hoon Hong, Won Jae Lee, Young Doo Kim, et al. "}, {"type": "b", "children": [{"type": "t", "text": "APIP, an ERBB3-binding partner, stimulates erbB2-3 heterodimer formation to promote tumorigenesis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Oncotarget (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.18632/oncotarget.7802"}], "href": "https://doi.org/10.18632/oncotarget.7802"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26942872"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26942872"}]}]}]}
Synonyms	CGI29, APIP2, CGI-29, MMRP19
Proteins	MTNB_HUMAN
NCBI Gene ID	51074
API
Download Associations
Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

APIP has 5,021 functional associations with biological entities spanning 9 categories (molecular profile, organism, chemical, functional term, phrase or reference, disease, phenotype or trait, structural feature, cell line, cell type or tissue, gene, protein or microRNA, sequence feature) extracted from 115 datasets.

Click the + buttons to view associations for APIP from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

APIP Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of APIP gene relative to other tissues from the Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles	tissues with high or low expression of APIP gene relative to other tissues from the Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles	tissue samples with high or low expression of APIP gene relative to other tissue samples from the Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of APIP gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq	tissue samples with high or low expression of APIP gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of APIP gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	BioGPS Cell Line Gene Expression Profiles	cell lines with high or low expression of APIP gene relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset.
	BioGPS Human Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of APIP gene relative to other cell types and tissues from the BioGPS Human Cell Type and Tissue Gene Expression Profiles dataset.
	BioGPS Mouse Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of APIP gene relative to other cell types and tissues from the BioGPS Mouse Cell Type and Tissue Gene Expression Profiles dataset.
	Carcinogenome Chemical Perturbation Carcinogenicity Signatures	small molecule perturbations changing expression of APIP gene from the Carcinogenome Chemical Perturbation Carcinogenicity Signatures dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of APIP gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Proteomics	Cell lines associated with APIP protein from the CCLE Cell Line Proteomics dataset.
	CellMarker Gene-Cell Type Associations	cell types associated with APIP gene from the CellMarker Gene-Cell Type Associations dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of APIP gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of APIP gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of APIP gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	CMAP Signatures of Differentially Expressed Genes for Small Molecules	small molecule perturbations changing expression of APIP gene from the CMAP Signatures of Differentially Expressed Genes for Small Molecules dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores	cellular components containing APIP protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores	cellular components co-occuring with APIP protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with APIP protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	COSMIC Cell Line Gene CNV Profiles	cell lines with high or low copy number of APIP gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with APIP gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Disease Associations	diseases associated with APIP gene/protein from the curated CTD Gene-Disease Associations dataset.
	dbGAP Gene-Trait Associations	traits associated with APIP gene in GWAS and other genetic association datasets from the dbGAP Gene-Trait Associations dataset.
	DepMap CRISPR Gene Dependency	cell lines with fitness changed by APIP gene knockdown relative to other cell lines from the DepMap CRISPR Gene Dependency dataset.
	DISEASES Experimental Gene-Disease Association Evidence Scores	diseases associated with APIP gene in GWAS datasets from the DISEASES Experimental Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Experimental Gene-Disease Association Evidence Scores 2025	diseases associated with APIP gene in GWAS datasets from the DISEASES Experimental Gene-Disease Assocation Evidence Scores 2025 dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with APIP gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores 2025	diseases co-occuring with APIP gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores 2025 dataset.
	DisGeNET Gene-Disease Associations	diseases associated with APIP gene in GWAS and other genetic association datasets from the DisGeNET Gene-Disease Associations dataset.
	DisGeNET Gene-Phenotype Associations	phenotypes associated with APIP gene in GWAS and other genetic association datasets from the DisGeNET Gene-Phenoptype Associations dataset.
	ENCODE Histone Modification Site Profiles	histone modification site profiles with high histone modification abundance at APIP gene from the ENCODE Histone Modification Site Profiles dataset.
	ENCODE Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of APIP gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
	ENCODE Transcription Factor Targets	transcription factors binding the promoter of APIP gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.
	ESCAPE Omics Signatures of Genes and Proteins for Stem Cells	PubMedIDs of publications reporting gene signatures containing APIP from the ESCAPE Omics Signatures of Genes and Proteins for Stem Cells dataset.
	GAD Gene-Disease Associations	diseases associated with APIP gene in GWAS and other genetic association datasets from the GAD Gene-Disease Associations dataset.
	GDSC Cell Line Gene Expression Profiles	cell lines with high or low expression of APIP gene relative to other cell lines from the GDSC Cell Line Gene Expression Profiles dataset.
	GeneRIF Biological Term Annotations	biological terms co-occuring with APIP gene in literature-supported statements describing functions of genes from the GeneRIF Biological Term Annotations dataset.
	GeneSigDB Published Gene Signatures	PubMedIDs of publications reporting gene signatures containing APIP from the GeneSigDB Published Gene Signatures dataset.
	GEO Signatures of Differentially Expressed Genes for Diseases	disease perturbations changing expression of APIP gene from the GEO Signatures of Differentially Expressed Genes for Diseases dataset.