{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nGas7, a multifaceted growth arrest‐specific protein, plays versatile roles in cell differentiation and oncogenic transformation. In hematopoietic cells, its deregulation—exemplified by fusion with the MLL gene—modulates progenitor homeostasis and influences leukemic disease latency and phenotype without being strictly required for transformation. In early embryonic stages, high Gas7 expression gives way to a dorsal restriction during neurulation, underscoring its participation in neuronal differentiation and migration, a disruption of which has been implicated in neurodevelopmental disorders such as schizophrenia."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "1", "end_ref": "3"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nIn the nervous system, Gas7 is critical for neuritogenesis and cytoskeletal organization. Overexpression in PC12 and primary neuronal cells promotes early morphological differentiation and stimulates NGF‐mediated neurite outgrowth, while its inhibition impairs the formation of neurite-like processes. Mechanistically, Gas7 interacts directly with N-WASP through unique WW-Pro domain interfaces to recruit the Arp2/3 complex for actin polymerization"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": ", and it binds Tau via its WW motif to facilitate microtubule assembly by stabilizing sheet intermediates."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "5"}]}, {"type": "t", "text": " In addition, Gas7 drives morphological differentiation in PC12 cells, contributing to the early events that precede and potentiate NGF-induced neuronal maturation."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "7"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nBeyond its roles in differentiation, Gas7 also regulates cell survival and immune functions. The Gas7b isoform, for instance, sensitizes neuroblastoma cells to apoptotic stimuli, while its depletion confers resistance to cell death under stress conditions."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "9"}]}, {"type": "t", "text": " In parallel, in macrophages Gas7 forms distinctive two-dimensional, sheet-like assemblies via its F-BAR domain that are indispensable for phagocytic cup formation during microbial internalization."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "10"}]}, {"type": "t", "text": " Furthermore, its induction in M1-polarized alveolar macrophages is regulated by NF-κB signaling and is linked to the expression of key pro-inflammatory genes."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "11"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Chi Wai So, Holger Karsunky, Piu Wong, et al. 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