{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n The cytosolic iron–sulfur assembly (CIA) machinery is responsible for inserting iron–sulfur (Fe–S) clusters into a wide spectrum of cytosolic and nuclear proteins that are essential for DNA replication, repair, telomere maintenance, and overall genome integrity. In this context, CIAO2B (also known as FAM96B) plays a pivotal role by partnering with CIA1 and MMS19 to form a targeting complex that facilitates the delivery and proper insertion of Fe–S clusters into most Fe–S proteins. This function is distinct from that of its paralog CIA2A—which specifically ensures the maturation of iron regulatory protein 1 (IRP1) involved in cellular iron homeostasis—by broadly supporting the maturation of proteins required for DNA metabolism and cell cycle progression. In addition to its role in Fe–S cluster assembly, CIAO2B is also important for maintaining mitotic fidelity and tolerance to replication stress; its downregulation has been associated with impaired cell cycle progression and increased sensitivity to agents that challenge DNA replication. Collectively, these activities underscore the essential contribution of CIAO2B in sustaining genomic stability and normal cell proliferation, with potential implications for tumorigenesis and other human diseases."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "1", "end_ref": "3"}]}, {"type": "t", "text": "\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Oliver Stehling, Judita Mascarenhas, Ajay A Vashisht, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Human CIA2A-FAM96A and CIA2B-FAM96B integrate iron homeostasis and maturation of different subsets of cytosolic-nuclear iron-sulfur proteins."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell Metab (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.cmet.2013.06.015"}], "href": "https://doi.org/10.1016/j.cmet.2013.06.015"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23891004"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23891004"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Lilach Ben-Shimon, Viktoria D Paul, Galit David-Kadoch, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Fe-S cluster coordination of the chromokinesin KIF4A alters its subcellular localization during mitosis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Cell Sci (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1242/jcs.211433"}], "href": "https://doi.org/10.1242/jcs.211433"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "29848660"}], "href": "https://pubmed.ncbi.nlm.nih.gov/29848660"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Abena B Redwood, Xiaomei Zhang, Sahil B Seth, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The cytosolic iron-sulfur cluster assembly (CIA) pathway is required for replication stress tolerance of cancer cells to Chk1 and ATR inhibitors."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "NPJ Breast Cancer (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41523-021-00353-2"}], "href": "https://doi.org/10.1038/s41523-021-00353-2"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34857765"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34857765"}]}]}]}