{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nTEX33, a testis-enriched protein, is exclusively expressed during mouse spermiogenesis, with its mRNA and protein first detected in the testes around postnatal days 21–28 and maintained into adulthood."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": " Detailed immunofluorescence analyses have localized TEX33 in developing spermatids and mature sperm, with a specific presence in the acrosome, flagellum, and manchette, suggesting a role in the structural organization during sperm formation. Despite these associations with spermatogenesis, genetic knockout experiments using CRISPR/Cas9 revealed that the absence of TEX33 does not alter testis-to-body weight ratios, sperm quality, or overall fertility, implying that while TEX33 is involved in the spermiogenic process, its function is likely non-essential or compensated for by redundant mechanisms during male reproduction.\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Mengmeng Xia, Jing Xia, Changmin Niu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Testis-expressed protein 33 is not essential for spermiogenesis and fertility in mice."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Med Rep (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3892/mmr.2021.11956"}], "href": "https://doi.org/10.3892/mmr.2021.11956"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "33760102"}], "href": "https://pubmed.ncbi.nlm.nih.gov/33760102"}]}]}]}