{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n Coronin 7 (CORO7) and PAM16 (also known as MAGMAS) are evolutionarily conserved proteins that contribute to distinct yet essential cellular functions. CORO7 is a unique member of the coronin family whose architecture—with dual WD‐repeat domains—differentiates it from other coronins that primarily modulate actin dynamics. Instead, CORO7 localizes predominantly to Golgi membranes where it orchestrates vesicle formation and cargo trafficking. Its recruitment to the Golgi depends on a sequential process involving Src kinase–mediated tyrosine phosphorylation and interactions with adaptor complexes such as AP‐1, thereby facilitating the budding of Golgi‐derived transport carriers and supporting localized actin assembly necessary for transport."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "1", "end_ref": "3"}]}, {"type": "t", "text": " Additionally, CORO7 functions as a scaffold protein within the Hippo signaling pathway by binding core pathway components and modulating protein–protein interactions that are pivotal for cell–cell contact responses and the regulation of organ size."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": " Its involvement in ubiquitin-dependent processes—such as forming part of an SCF-like complex that promotes the degradation of regulatory proteins—further highlights its multifaceted role in intracellular trafficking and signal transduction.\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n In contrast, PAM16 is a mitochondria-associated protein that plays a critical role in the translocation of nuclear-encoded preproteins into the mitochondrial matrix. Expressed in trabecular bone and cartilage during early development, PAM16 is indispensable for normal ossification. Mutations that destabilize PAM16 impair mitochondrial protein import, triggering cell death and leading to severe skeletal dysplasias."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "5"}]}, {"type": "t", "text": "\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n In summary, while CORO7 is central to the organization of intracellular membrane compartments and the regulation of post-Golgi vesicular trafficking as well as participating in signal transduction pathways like Hippo, PAM16 is essential for mitochondrial protein import and energy homeostasis during development. Together, these studies underscore how evolutionarily conserved proteins achieve cellular specificity by engaging in discrete protein networks that control critical aspects of cellular physiology.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "V Rybakin, R H Rastetter, M Stumpf, et al. 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"}, {"type": "b", "children": [{"type": "t", "text": "Coronin 7, the mammalian POD-1 homologue, localizes to the Golgi apparatus."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "FEBS Lett (2004)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.febslet.2004.07.066"}], "href": "https://doi.org/10.1016/j.febslet.2004.07.066"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "15327992"}], "href": "https://pubmed.ncbi.nlm.nih.gov/15327992"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Wei-Chien Yuan, Yu-Ru Lee, Shu-Yu Lin, et al. "}, {"type": "b", "children": [{"type": "t", "text": "K33-Linked Polyubiquitination of Coronin 7 by Cul3-KLHL20 Ubiquitin E3 Ligase Regulates Protein Trafficking."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Cell (2014)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.molcel.2014.03.035"}], "href": "https://doi.org/10.1016/j.molcel.2014.03.035"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "24768539"}], "href": "https://pubmed.ncbi.nlm.nih.gov/24768539"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Jina Park, Kyoungho Jun, Yujin Choi, et al. "}, {"type": "b", "children": [{"type": "t", "text": "CORO7 functions as a scaffold protein for the core kinase complex assembly of the Hippo pathway."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.RA120.013297"}], "href": "https://doi.org/10.1074/jbc.RA120.013297"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "33162394"}], "href": "https://pubmed.ncbi.nlm.nih.gov/33162394"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Cybel Mehawej, Agnès Delahodde, Laurence Legeai-Mallet, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The impairment of MAGMAS function in human is responsible for a severe skeletal dysplasia."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS Genet (2014)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pgen.1004311"}], "href": "https://doi.org/10.1371/journal.pgen.1004311"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "24786642"}], "href": "https://pubmed.ncbi.nlm.nih.gov/24786642"}]}]}]}