{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n The CPLANE complex (ciliogenesis and planar polarity effector) plays a critical role in the assembly and maintenance of primary cilia by integrating planar cell polarity cues with vesicular trafficking and intraflagellar transport. Composed of proteins such as Inturned, Fuzzy, WDPCP, JBTS17, and the small GTPase Rsg1, the complex orchestrates multiple steps in ciliogenesis. Detailed structural studies show that the CPLANE complex adopts a crescent‐shaped architecture that mediates binding to phospholipids and harbors homologous longin domains—features that implicate the complex in acting as a Rab guanine nucleotide exchange factor to activate Rab GTPases involved in vesicular trafficking. Functionally, CPLANE facilitates the recruitment and proper localization of intraflagellar transport components at the basal body, thereby ensuring efficient ciliary vesicle docking, axoneme elongation, and overall cilia function. Disruption of CPLANE components leads to defective intraflagellar transport dynamics, mislocalized basal bodies, and ultimately to ciliopathies that manifest with developmental anomalies and impaired cell signaling."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "1", "end_ref": "3"}]}, {"type": "t", "text": "\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Gerasimos Langousis, Simone Cavadini, Niels Boegholm, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Structure of the ciliogenesis-associated CPLANE complex."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Sci Adv (2022)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1126/sciadv.abn0832"}], "href": "https://doi.org/10.1126/sciadv.abn0832"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "35427153"}], "href": "https://pubmed.ncbi.nlm.nih.gov/35427153"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Luis Sanchez-Pulido, Chris P Ponting "}, {"type": "b", "children": [{"type": "t", "text": "Hexa-Longin domain scaffolds for inter-Rab signalling."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Bioinformatics (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1093/bioinformatics/btz739"}], "href": "https://doi.org/10.1093/bioinformatics/btz739"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "31562761"}], "href": "https://pubmed.ncbi.nlm.nih.gov/31562761"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Michinori Toriyama, Chanjae Lee, S Paige Taylor, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The ciliopathy-associated CPLANE proteins direct basal body recruitment of intraflagellar transport machinery."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Genet (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/ng.3558"}], "href": "https://doi.org/10.1038/ng.3558"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "27158779"}], "href": "https://pubmed.ncbi.nlm.nih.gov/27158779"}]}]}]}