{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nCRYBG1, also known as AIM1, is a member of the βγ‐crystallin superfamily that contains multiple conserved crystallin motifs. Structural studies of its variant domain (AIM1g1) have shown that despite significant sequence divergence from canonical βγ‐crystallins, CRYBG1 retains the overall Greek key fold and exhibits unique surface loop features that facilitate calcium binding. These adaptations may provide the structural basis for its novel biological roles."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nIn the context of cancer, CRYBG1/AIM1 has been implicated as a tumor suppressor, particularly in melanoma. Genetic alterations—including deletions and promoter hypermethylation—have been correlated with diminished expression of CRYBG1 in aggressive malignancies. Epigenetic modifications affecting CRYBG1 accompany similar deregulation of other cell cycle regulators and transcription factors, suggesting that its loss or inactivation contributes to tumorigenesis and disease progression."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "3", "end_ref": "5"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nBeyond melanoma, altered CRYBG1 expression has also been observed in prostate cancers, where integrated gene expression and RNAi screening studies support its involvement in modulating oncogenic pathways and cellular survival. Furthermore, investigations into the mechanistic basis of its function reveal that calcium-dependent conformational dynamics and the interplay between its structural domains can compensate for potential instabilities, thereby maintaining proper protein function. These findings underscore the multifaceted role of CRYBG1 in both structure–function relationships and tumor biology."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "6"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Penmatsa Aravind, Bheemreddy Rajini, Yogendra Sharma, et al. 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