CSTF2T Gene

HGNC Family	RNA binding motif containing (RBM)
Name	cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa, tau variant
Description	Enables RNA binding activity. Predicted to be involved in mRNA 3'-end processing. Located in nucleoplasm. [provided by Alliance of Genome Resources, Mar 2025]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n The CSTF2T gene encodes tauCstF‐64, a specialized paralog of the canonical cleavage stimulation factor CstF‐64 that plays a critical role in mRNA 3′ end formation. In most somatic cells, CstF‐64 mediates cleavage/polyadenylation at transcripts bearing conserved downstream sequence elements; however, during male meiosis the X‐linked CstF‐64 is silenced, and tauCstF‐64 takes over to direct alternative polyadenylation in germ cells. In this context, tauCstF‐64 ensures proper selection of polyadenylation sites, thereby promoting correct 3′ end processing of transcripts essential for spermatogenesis. Studies in mouse knockout models have demonstrated that loss of CSTF2T results in widespread changes in mRNA 3′ end formation, aberrant transcription termination, and defects in both meiotic and postmeiotic germ cell development that manifest as male infertility, including impaired sperm morphology and decreased sperm–zona pellucida adhesion."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "1", "end_ref": "3"}]}, {"type": "t", "text": " At the molecular level, tauCstF‐64 exhibits RNA‐binding properties that differ from those of its somatic counterpart, favoring recognition of distinct downstream sequence elements; such differential binding contributes to a germ cell–specific pattern of alternative polyadenylation, thereby modulating the expression of thousands of testicular genes."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": " Moreover, the cloning and mapping of the human ortholog of CSTF2T have established its association with neurological phenotypes and male infertility, and its lower level but detectable expression in the brain suggests an extended role in neural RNA processing."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "6", "end_ref": "8"}]}, {"type": "t", "text": "\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n Together, these findings position CSTF2T/tauCstF‐64 as a key regulator of alternative polyadenylation that safeguards proper gene expression during male germ cell development and potentially in neural tissues, thereby impacting both reproductive function and broader aspects of cellular differentiation.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Brinda Dass, Steve Tardif, Ji Yeon Park, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Loss of polyadenylation protein tauCstF-64 causes spermatogenic defects and male infertility."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Proc Natl Acad Sci U S A (2007)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1073/pnas.0707589104"}], "href": "https://doi.org/10.1073/pnas.0707589104"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "18077340"}], "href": "https://pubmed.ncbi.nlm.nih.gov/18077340"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Steve Tardif, Amma S Akrofi, Brinda Dass, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Infertility with impaired zona pellucida adhesion of spermatozoa from mice lacking TauCstF-64."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biol Reprod (2010)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1095/biolreprod.109.083238"}], "href": "https://doi.org/10.1095/biolreprod.109.083238"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "20463354"}], "href": "https://pubmed.ncbi.nlm.nih.gov/20463354"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Kathy Jo Hockert, Kathleen Martincic, S M L C Mendis-Handagama, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Spermatogenetic but not immunological defects in mice lacking the τCstF-64 polyadenylation protein."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Reprod Immunol (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.jri.2011.01.018"}], "href": "https://doi.org/10.1016/j.jri.2011.01.018"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21489638"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21489638"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Wencheng Li, Hsiang-Jui Yeh, Ganesh S Shankarling, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The τCstF-64 polyadenylation protein controls genome expression in testis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS One (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pone.0048373"}], "href": "https://doi.org/10.1371/journal.pone.0048373"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23110235"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23110235"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Roberto R Monarez, Clinton C MacDonald, Brinda Dass "}, {"type": "b", "children": [{"type": "t", "text": "Polyadenylation proteins CstF-64 and tauCstF-64 exhibit differential binding affinities for RNA polymers."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biochem J (2007)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1042/BJ20061097"}], "href": "https://doi.org/10.1042/BJ20061097"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "17029590"}], "href": "https://pubmed.ncbi.nlm.nih.gov/17029590"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Brinda Dass, Lisa McDaniel, Roger A Schultz, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The gene CSTF2T, encoding the human variant CstF-64 polyadenylation protein tauCstF-64, lacks introns and may be associated with male sterility."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Genomics (2002)"}]}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "12408968"}], "href": "https://pubmed.ncbi.nlm.nih.gov/12408968"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Jaryse C Harris, Joseph M Martinez, Petar N Grozdanov, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The Cstf2t Polyadenylation Gene Plays a Sex-Specific Role in Learning Behaviors in Mice."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS One (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pone.0165976"}], "href": "https://doi.org/10.1371/journal.pone.0165976"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "27812195"}], "href": "https://pubmed.ncbi.nlm.nih.gov/27812195"}]}, {"type": "r", "ref": 8, "children": [{"type": "t", "text": "A Michelle Wallace, Toni L Denison, Ebtesam N Attaya, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Developmental distribution of the polyadenylation protein CstF-64 and the variant tauCstF-64 in mouse and rat testis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biol Reprod (2004)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1095/biolreprod.103.022947"}], "href": "https://doi.org/10.1095/biolreprod.103.022947"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "14681198"}], "href": "https://pubmed.ncbi.nlm.nih.gov/14681198"}]}]}]}
Synonyms	CSTF-64T
Proteins	CSTFT_HUMAN
NCBI Gene ID	23283
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Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

CSTF2T has 5,774 functional associations with biological entities spanning 8 categories (molecular profile, organism, chemical, functional term, phrase or reference, disease, phenotype or trait, structural feature, cell line, cell type or tissue, gene, protein or microRNA) extracted from 117 datasets.

Click the + buttons to view associations for CSTF2T from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

CSTF2T Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of CSTF2T gene relative to other tissues from the Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles	tissues with high or low expression of CSTF2T gene relative to other tissues from the Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles	tissue samples with high or low expression of CSTF2T gene relative to other tissue samples from the Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of CSTF2T gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq	tissue samples with high or low expression of CSTF2T gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of CSTF2T gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	BioGPS Cell Line Gene Expression Profiles	cell lines with high or low expression of CSTF2T gene relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset.
	BioGPS Human Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of CSTF2T gene relative to other cell types and tissues from the BioGPS Human Cell Type and Tissue Gene Expression Profiles dataset.
	BioGPS Mouse Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of CSTF2T gene relative to other cell types and tissues from the BioGPS Mouse Cell Type and Tissue Gene Expression Profiles dataset.
	Carcinogenome Chemical Perturbation Carcinogenicity Signatures	small molecule perturbations changing expression of CSTF2T gene from the Carcinogenome Chemical Perturbation Carcinogenicity Signatures dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of CSTF2T gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Gene Expression Profiles	cell lines with high or low expression of CSTF2T gene relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.
	CCLE Cell Line Proteomics	Cell lines associated with CSTF2T protein from the CCLE Cell Line Proteomics dataset.
	CellMarker Gene-Cell Type Associations	cell types associated with CSTF2T gene from the CellMarker Gene-Cell Type Associations dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of CSTF2T gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of CSTF2T gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of CSTF2T gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	CM4AI U2OS Cell Map Protein Localization Assemblies	assemblies containing CSTF2T protein from integrated AP-MS and IF data from the CM4AI U2OS Cell Map Protein Localization Assemblies dataset.
	CMAP Signatures of Differentially Expressed Genes for Small Molecules	small molecule perturbations changing expression of CSTF2T gene from the CMAP Signatures of Differentially Expressed Genes for Small Molecules dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores	cellular components containing CSTF2T protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores 2025	cellular components containing CSTF2T protein from the COMPARTMENTS Curated Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores	cellular components containing CSTF2T protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores 2025	cellular components containing CSTF2T protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores	cellular components co-occuring with CSTF2T protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with CSTF2T protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	COSMIC Cell Line Gene CNV Profiles	cell lines with high or low copy number of CSTF2T gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with CSTF2T gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Chemical Interactions	chemicals interacting with CSTF2T gene/protein from the curated CTD Gene-Chemical Interactions dataset.
	CTD Gene-Disease Associations	diseases associated with CSTF2T gene/protein from the curated CTD Gene-Disease Associations dataset.
	DeepCoverMOA Drug Mechanisms of Action	small molecule perturbations with high or low expression of CSTF2T protein relative to other small molecule perturbations from the DeepCoverMOA Drug Mechanisms of Action dataset.
	DepMap CRISPR Gene Dependency	cell lines with fitness changed by CSTF2T gene knockdown relative to other cell lines from the DepMap CRISPR Gene Dependency dataset.
	DISEASES Experimental Gene-Disease Association Evidence Scores 2025	diseases associated with CSTF2T gene in GWAS datasets from the DISEASES Experimental Gene-Disease Assocation Evidence Scores 2025 dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with CSTF2T gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores 2025	diseases co-occuring with CSTF2T gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores 2025 dataset.
	DisGeNET Gene-Phenotype Associations	phenotypes associated with CSTF2T gene in GWAS and other genetic association datasets from the DisGeNET Gene-Phenoptype Associations dataset.
	ENCODE Histone Modification Site Profiles	histone modification site profiles with high histone modification abundance at CSTF2T gene from the ENCODE Histone Modification Site Profiles dataset.
	ENCODE Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of CSTF2T gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
	ENCODE Transcription Factor Targets	transcription factors binding the promoter of CSTF2T gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.
	ESCAPE Omics Signatures of Genes and Proteins for Stem Cells	PubMedIDs of publications reporting gene signatures containing CSTF2T from the ESCAPE Omics Signatures of Genes and Proteins for Stem Cells dataset.
	GAD High Level Gene-Disease Associations	diseases associated with CSTF2T gene in GWAS and other genetic association datasets from the GAD High Level Gene-Disease Associations dataset.