{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n C1orf123 (referred to here as CZIB) is an evolutionarily conserved, yet still poorly understood protein that belongs to the DUF866 family. Structural and biochemical studies have revealed that CZIB harbors two conserved CxxC motifs that mediate tetrahedral zinc coordination, suggesting a role in metal ion binding and potentially in the regulation of metal‐dependent protein–protein interactions."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": " Functional analyses indicate that CZIB interacts specifically with metal ion chaperones – for example, it has been shown to interact with a copper chaperone domain through its CxxC regions, implicating it in intracellular metal trafficking and homeostasis."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": " Moreover, proteomic screenings in specialized tissues, such as the electric organ of Torpedo californica, have identified CZIB as one of the proteins with high sequence similarity to components of neuromuscular junctions, hinting at a potential role in synaptic or muscle-specific processes."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": " In addition, differential expression profiles in human synovial membranes among various arthritic conditions have revealed that CZIB expression correlates with inflammation, suggesting it may serve as a molecular marker in inflammatory responses at the synapse or in muscle tissue."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": " Collectively, while the full spectrum of its cellular functions remains to be elucidated, current evidence supports a model in which CZIB contributes to metal ion regulation and may participate in specialized signaling complexes at synaptic or muscle-related sites.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Yoshiaki Furukawa, Carolyn Lim, Takehiko Tosha, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Identification of a novel zinc-binding protein, C1orf123, as an interactor with a heavy metal-associated domain."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS One (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pone.0204355"}], "href": "https://doi.org/10.1371/journal.pone.0204355"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30260988"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30260988"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Siti Nurulnabila A Rahaman, Jastina Mat Yusop, Zeti-Azura Mohamed-Hussein, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Cloning, expression, purification, crystallization and X-ray crystallographic analysis of recombinant human C1ORF123 protein."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Acta Crystallogr F Struct Biol Commun (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1107/S2053230X16002016"}], "href": "https://doi.org/10.1107/S2053230X16002016"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26919524"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26919524"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Suzanne E Mate, Kristy J Brown, Eric P Hoffman "}, {"type": "b", "children": [{"type": "t", "text": "Integrated genomics and proteomics of the Torpedo californica electric organ: concordance with the mammalian neuromuscular junction."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Skelet Muscle (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1186/2044-5040-1-20"}], "href": "https://doi.org/10.1186/2044-5040-1-20"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21798097"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21798097"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Dominique de Seny, Dominique Baiwir, Elettra Bianchi, et al. "}, {"type": "b", "children": [{"type": "t", "text": "New Proteins Contributing to Immune Cell Infiltration and Pannus Formation of Synovial Membrane from Arthritis Diseases."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Int J Mol Sci (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3390/ijms23010434"}], "href": "https://doi.org/10.3390/ijms23010434"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "35008858"}], "href": "https://pubmed.ncbi.nlm.nih.gov/35008858"}]}]}]}