{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nRecent investigations into C2C12 myogenic differentiation have revealed that LRRC75B (also known as AI646023) acts as a novel suppressor of muscle cell development. Specifically, silencing LRRC75B using siRNA in C2C12 myoblasts significantly enhanced the expression of the muscle‐specific transcription factor myogenin, which in turn promoted increased myoblast fusion and a greater diameter of myotubes. Conversely, overexpression of LRRC75B markedly inhibited myogenic differentiation, an effect accompanied by reduced myogenin expression and a suppression of extracellular signal-regulated kinase 1/2 (Erk1/2) phosphorylation. Together, these findings suggest that LRRC75B modulates myogenic differentiation through a mechanism that regulates both myogenin expression and Erk1/2 signaling, thereby defining it as a critical negative regulator of muscle cell formation."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Yuechun Zhong, Liyi Zou, Zonggui Wang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Lrrc75b is a novel negative regulator of C2C12 myogenic differentiation."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Int J Mol Med (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3892/ijmm.2016.2738"}], "href": "https://doi.org/10.3892/ijmm.2016.2738"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "27633041"}], "href": "https://pubmed.ncbi.nlm.nih.gov/27633041"}]}]}]}