{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n DEUP1 is emerging as a master regulator of the deuterosome‐dependent pathway for centriole amplification in multiciliated cells. In contrast to the mother centriole–dependent duplication pathway (mediated by proteins such as CEP63), DEUP1 governs the assembly of non‐membranous deuterosomes that serve as platforms for the de novo biogenesis of the hundreds of centrioles required to nucleate motile cilia. DEUP1 exerts its function by binding to key centrosomal factors – for example, it interacts with CEP152 and recruits the kinase Plk4 to activate the centriole duplication machinery, thereby initiating large‐scale centriole assembly."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": " Recent studies further indicate that DEUP1 has the intrinsic ability to self‐assemble into macromolecular condensates, a property that likely underlies its capacity to nucleate the formation of deuterosomes."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": " Moreover, transcriptional programs driving multiciliogenesis specifically activate DEUP1 expression – for instance, the Multicilin/E2F4 complex selectively upregulates DEUP1 (but not its paralog CEP63), thereby shifting centriole duplication from a templated to a de novo mechanism"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": ";."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": " Collectively, these findings position DEUP1 as a critical factor that not only orchestrates the assembly of deuterosomes but also ensures the robust production of centrioles necessary for the formation of multiple motile cilia in diverse tissues.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Huijie Zhao, Lei Zhu, Yunlu Zhu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Cell Biol (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/ncb2880"}], "href": "https://doi.org/10.1038/ncb2880"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "24240477"}], "href": "https://pubmed.ncbi.nlm.nih.gov/24240477"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Shohei Yamamoto, Ryoichi Yabuki, Daiju Kitagawa "}, {"type": "b", "children": [{"type": "t", "text": "Biophysical and biochemical properties of Deup1 self-assemblies: a potential driver for deuterosome formation during multiciliogenesis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biol Open (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1242/bio.056432"}], "href": "https://doi.org/10.1242/bio.056432"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "33658185"}], "href": "https://pubmed.ncbi.nlm.nih.gov/33658185"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Renin Hazan, Munemasa Mori, Paul S Danielian, et al. "}, {"type": "b", "children": [{"type": "t", "text": "E2F4's cytoplasmic role in multiciliogenesis is mediated via an N-terminal domain that binds two components of the centriole replication machinery, Deup1 and SAS6."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Biol Cell (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1091/mbc.E21-01-0039"}], "href": "https://doi.org/10.1091/mbc.E21-01-0039"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34260288"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34260288"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Lina Ma, Ian Quigley, Heymut Omran, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Multicilin drives centriole biogenesis via E2f proteins."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Genes Dev (2014)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1101/gad.243832.114"}], "href": "https://doi.org/10.1101/gad.243832.114"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "24934224"}], "href": "https://pubmed.ncbi.nlm.nih.gov/24934224"}]}]}]}