E2F3 Gene

Name	E2F transcription factor 3
Description	This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
Summary	{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n E2F3 is a key member of the E2F transcription factor family that acts as a potent transcriptional activator to drive cell cycle progression—particularly the G1/S transition—and thereby promotes DNA replication and cell proliferation (7). Several studies have highlighted that the deregulated expression of E2F3 contributes to oncogenic progression. For example, in neuroblastoma, miR‐34a directly targets the E2F3 messenger RNA and reduces its protein levels, which leads to decreased cell proliferation and the induction of caspase‐dependent apoptosis (1). In addition, regulation of E2F3 translation by miR‐20a from the mir‐17–92 cluster establishes an autoregulatory feedback loop that carefully modulates the accumulation of E2F activators, ensuring proper cell cycle control (2)."}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n In hypoxic conditions, stabilization of hypoxia‐inducible factor (HIF) leads to the upregulation of miR‐210, which in turn downregulates E2F3 expression. This mechanism links low oxygen tension to impaired cell cycle progression in contexts such as ischemic wound healing (3,6). Furthermore, in non‐small cell lung cancer, the long noncoding RNA NEAT1 functions as a competing endogenous RNA to sequester miR‐377–3p. This interaction de-represses E2F3, thereby enhancing its oncogenic activity and contributing to tumor progression (4). In retinoblastoma, increased levels of E2F3 are observed during the progression from stable, senescent retinal lesions to highly proliferative malignant tumors, underscoring its critical role in tumorigenesis (5).\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n Collectively, these findings demonstrate that E2F3 is central to the regulation of cell cycle progression under both normal and stress conditions. Its tight regulation by microRNAs and long noncoding RNAs is crucial for maintaining cellular homeostasis, while its overexpression or de‐regulation can drive oncogenic processes in diverse cancers.\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n "}, {"type": "b", "children": [{"type": "t", "text": "Citations:"}]}, {"type": "t", "text": "<br>\n (1)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ", \n (2)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": ", \n (3)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": ", \n (4)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": ", \n (5)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "5"}]}, {"type": "t", "text": ", \n (6)"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "6"}]}, {"type": "t", "text": ", \n (7)."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "7"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "C Welch, Y Chen, R L Stallings "}, {"type": "b", "children": [{"type": "t", "text": "MicroRNA-34a functions as a potential tumor suppressor by inducing apoptosis in neuroblastoma cells."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Oncogene (2007)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/sj.onc.1210293"}], "href": "https://doi.org/10.1038/sj.onc.1210293"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "17297439"}], "href": "https://pubmed.ncbi.nlm.nih.gov/17297439"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Yannick Sylvestre, Vincent De Guire, Emmanuelle Querido, et al. "}, {"type": "b", "children": [{"type": "t", "text": "An E2F/miR-20a autoregulatory feedback loop."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Biol Chem (2007)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1074/jbc.M608939200"}], "href": "https://doi.org/10.1074/jbc.M608939200"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "17135249"}], "href": "https://pubmed.ncbi.nlm.nih.gov/17135249"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Antonis Giannakakis, Raphael Sandaltzopoulos, Joel Greshock, et al. "}, {"type": "b", "children": [{"type": "t", "text": "miR-210 links hypoxia with cell cycle regulation and is deleted in human epithelial ovarian cancer."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cancer Biol Ther (2008)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.4161/cbt.7.2.5297"}], "href": "https://doi.org/10.4161/cbt.7.2.5297"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "18059191"}], "href": "https://pubmed.ncbi.nlm.nih.gov/18059191"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Chengcao Sun, Shujun Li, Feng Zhang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Long non-coding RNA NEAT1 promotes non-small cell lung cancer progression through regulation of miR-377-3p-E2F3 pathway."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Oncotarget (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.18632/oncotarget.10108"}], "href": "https://doi.org/10.18632/oncotarget.10108"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "27351135"}], "href": "https://pubmed.ncbi.nlm.nih.gov/27351135"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Helen Dimaras, Vikas Khetan, William Halliday, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Loss of RB1 induces non-proliferative retinoma: increasing genomic instability correlates with progression to retinoblastoma."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Hum Mol Genet (2008)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1093/hmg/ddn024"}], "href": "https://doi.org/10.1093/hmg/ddn024"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "18211953"}], "href": "https://pubmed.ncbi.nlm.nih.gov/18211953"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Sabyasachi Biswas, Sashwati Roy, Jaideep Banerjee, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Hypoxia inducible microRNA 210 attenuates keratinocyte proliferation and impairs closure in a murine model of ischemic wounds."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Proc Natl Acad Sci U S A (2010)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1073/pnas.1001653107"}], "href": "https://doi.org/10.1073/pnas.1001653107"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "20308562"}], "href": "https://pubmed.ncbi.nlm.nih.gov/20308562"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "L Wu, C Timmers, B Maiti, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The E2F1-3 transcription factors are essential for cellular proliferation."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nature (2001)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/35106593"}], "href": "https://doi.org/10.1038/35106593"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "11719808"}], "href": "https://pubmed.ncbi.nlm.nih.gov/11719808"}]}]}]}
Synonyms	E2F-3
Proteins	E2F3_HUMAN
NCBI Gene ID	1871
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Predicted Functions
Co-expressed Genes
Expression in Tissues and Cell Lines

Functional Associations

E2F3 has 9,469 functional associations with biological entities spanning 8 categories (molecular profile, organism, chemical, functional term, phrase or reference, disease, phenotype or trait, structural feature, cell line, cell type or tissue, gene, protein or microRNA) extracted from 133 datasets.

Click the + buttons to view associations for E2F3 from the datasets below.

If available, associations are ranked by standardized value

Harmonizome 3.0

E2F3 Gene

Functional Associations

Please acknowledge the Harmonizome in your publications by citing the following reference(s):

	Dataset	Summary
	Achilles Cell Line Gene Essentiality Profiles	cell lines with fitness changed by E2F3 gene knockdown relative to other cell lines from the Achilles Cell Line Gene Essentiality Profiles dataset.
	Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of E2F3 gene relative to other tissues from the Allen Brain Atlas Adult Human Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles	tissues with high or low expression of E2F3 gene relative to other tissues from the Allen Brain Atlas Adult Mouse Brain Tissue Gene Expression Profiles dataset.
	Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles	tissue samples with high or low expression of E2F3 gene relative to other tissue samples from the Allen Brain Atlas Aging Dementia and Traumatic Brain Injury Tissue Sample Gene Expression Profiles dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray	tissue samples with high or low expression of E2F3 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by Microarray dataset.
	Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq	tissue samples with high or low expression of E2F3 gene relative to other tissue samples from the Allen Brain Atlas Developing Human Brain Tissue Gene Expression Profiles by RNA-seq dataset.
	Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles	tissues with high or low expression of E2F3 gene relative to other tissues from the Allen Brain Atlas Prenatal Human Brain Tissue Gene Expression Profiles dataset.
	BioGPS Cell Line Gene Expression Profiles	cell lines with high or low expression of E2F3 gene relative to other cell lines from the BioGPS Cell Line Gene Expression Profiles dataset.
	BioGPS Human Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of E2F3 gene relative to other cell types and tissues from the BioGPS Human Cell Type and Tissue Gene Expression Profiles dataset.
	BioGPS Mouse Cell Type and Tissue Gene Expression Profiles	cell types and tissues with high or low expression of E2F3 gene relative to other cell types and tissues from the BioGPS Mouse Cell Type and Tissue Gene Expression Profiles dataset.
	Carcinogenome Chemical Perturbation Carcinogenicity Signatures	small molecule perturbations changing expression of E2F3 gene from the Carcinogenome Chemical Perturbation Carcinogenicity Signatures dataset.
	CCLE Cell Line Gene CNV Profiles	cell lines with high or low copy number of E2F3 gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
	CCLE Cell Line Gene Expression Profiles	cell lines with high or low expression of E2F3 gene relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset.
	CCLE Cell Line Proteomics	Cell lines associated with E2F3 protein from the CCLE Cell Line Proteomics dataset.
	ChEA Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of E2F3 gene from the CHEA Transcription Factor Binding Site Profiles dataset.
	ChEA Transcription Factor Targets	transcription factors binding the promoter of E2F3 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets dataset.
	ChEA Transcription Factor Targets 2022	transcription factors binding the promoter of E2F3 gene in low- or high-throughput transcription factor functional studies from the CHEA Transcription Factor Targets 2022 dataset.
	CM4AI KOLF21J CRISPRi Gene Perturbation Atlas	gene perturbations changing expression of E2F3 gene from the CM4AI KOLF21J CRISPRi Gene Perturbation Atlas dataset.
	CMAP Signatures of Differentially Expressed Genes for Small Molecules	small molecule perturbations changing expression of E2F3 gene from the CMAP Signatures of Differentially Expressed Genes for Small Molecules dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores	cellular components containing E2F3 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores dataset.
	COMPARTMENTS Curated Protein Localization Evidence Scores 2025	cellular components containing E2F3 protein from the COMPARTMENTS Curated Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Experimental Protein Localization Evidence Scores 2025	cellular components containing E2F3 protein in low- or high-throughput protein localization assays from the COMPARTMENTS Experimental Protein Localization Evidence Scores 2025 dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores	cellular components co-occuring with E2F3 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores dataset.
	COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025	cellular components co-occuring with E2F3 protein in abstracts of biomedical publications from the COMPARTMENTS Text-mining Protein Localization Evidence Scores 2025 dataset.
	COSMIC Cell Line Gene CNV Profiles	cell lines with high or low copy number of E2F3 gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset.
	COSMIC Cell Line Gene Mutation Profiles	cell lines with E2F3 gene mutations from the COSMIC Cell Line Gene Mutation Profiles dataset.
	CTD Gene-Chemical Interactions	chemicals interacting with E2F3 gene/protein from the curated CTD Gene-Chemical Interactions dataset.
	CTD Gene-Disease Associations	diseases associated with E2F3 gene/protein from the curated CTD Gene-Disease Associations dataset.
	dbGAP Gene-Trait Associations	traits associated with E2F3 gene in GWAS and other genetic association datasets from the dbGAP Gene-Trait Associations dataset.
	DeepCoverMOA Drug Mechanisms of Action	small molecule perturbations with high or low expression of E2F3 protein relative to other small molecule perturbations from the DeepCoverMOA Drug Mechanisms of Action dataset.
	DepMap CRISPR Gene Dependency	cell lines with fitness changed by E2F3 gene knockdown relative to other cell lines from the DepMap CRISPR Gene Dependency dataset.
	DGIdb Drug Targets 2026	interacting drugs for E2F3 protein from the DGIdb Drug Targets 2026 dataset.
	DISEASES Experimental Gene-Disease Association Evidence Scores 2025	diseases associated with E2F3 gene in GWAS datasets from the DISEASES Experimental Gene-Disease Assocation Evidence Scores 2025 dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores	diseases co-occuring with E2F3 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores dataset.
	DISEASES Text-mining Gene-Disease Association Evidence Scores 2025	diseases co-occuring with E2F3 gene in abstracts of biomedical publications from the DISEASES Text-mining Gene-Disease Assocation Evidence Scores 2025 dataset.
	DisGeNET Gene-Disease Associations	diseases associated with E2F3 gene in GWAS and other genetic association datasets from the DisGeNET Gene-Disease Associations dataset.
	DisGeNET Gene-Phenotype Associations	phenotypes associated with E2F3 gene in GWAS and other genetic association datasets from the DisGeNET Gene-Phenoptype Associations dataset.
	ENCODE Histone Modification Site Profiles	histone modification site profiles with high histone modification abundance at E2F3 gene from the ENCODE Histone Modification Site Profiles dataset.
	ENCODE Transcription Factor Binding Site Profiles	transcription factor binding site profiles with transcription factor binding evidence at the promoter of E2F3 gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
	ENCODE Transcription Factor Targets	transcription factors binding the promoter of E2F3 gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.