{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nEEIG1 is an early estrogen-induced gene that was first identified in estrogen receptor–positive breast cancer cells. It is rapidly upregulated—within 2 hours—following treatment with 17β-estradiol and a variety of other estrogenic compounds. Its induction is selectively observed in ER‐positive cells and is sensitive to blockade by antiestrogens, suggesting that EEIG1 functions as a primary transcriptional target in estrogen signaling pathways."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nIn addition to its role in estrogen response, EEIG1 has been identified as a critical signaling component in osteoclastogenesis. Upon stimulation by RANK ligand, EEIG1 physically interacts with the RANK receptor and further associates with adaptors and kinases such as Gab2, PLCγ2, and Tec/Btk to promote PLCγ2 phosphorylation and NFATc1 induction. These interactions facilitate osteoclast formation and bone resorption, with inhibition of the RANK–EEIG1 interaction markedly reducing osteoclast activity and subsequent bone destruction—suggesting that EEIG1 may represent a promising therapeutic target in pathological bone resorption."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Dong-Yu Wang, Roberta Fulthorpe, Steven N Liss, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Identification of estrogen-responsive genes by complementary deoxyribonucleic acid microarray and characterization of a novel early estrogen-induced gene: EEIG1."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Endocrinol (2004)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1210/me.2003-0202"}], "href": "https://doi.org/10.1210/me.2003-0202"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "14605097"}], "href": "https://pubmed.ncbi.nlm.nih.gov/14605097"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Han Kyoung Choi, Hye Ri Kang, Eutteum Jung, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Early estrogen-induced gene 1, a novel RANK signaling component, is essential for osteoclastogenesis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell Res (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/cr.2013.33"}], "href": "https://doi.org/10.1038/cr.2013.33"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "23478294"}], "href": "https://pubmed.ncbi.nlm.nih.gov/23478294"}]}]}]}