{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n Endothelial‐overexpressed lipopolysaccharide‐associated factor 1 (EOLA1) is an intracellular protein that plays a multifaceted role in vascular endothelial cell homeostasis. It is rapidly induced by lipopolysaccharide (LPS) stimulation and, through its physical interaction with metallothionein 2A (MT2A), acts as a negative regulator of inflammatory responses by suppressing the production of pro‐inflammatory mediators such as interleukin‑6 and vascular cell adhesion molecule‑1, and by protecting cells from LPS‐induced apoptosis."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": " Moreover, EOLA1 influences endothelial cell proliferation as its overexpression enhances cell growth whereas its inhibition slows proliferation."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": " Molecular cloning studies have revealed that EOLA1 is encoded by a 1404‐bp transcript producing a 158–amino acid protein that localizes to both the nucleus and the cytoplasmic matrix, consistent with its function as a signal transduction factor."}, {"type": "fg", "children": [{"type": "fg_fs", "start_ref": "5", "end_ref": "7"}]}, {"type": "t", "text": " Its role also appears pertinent in pathological settings such as diabetic foot ulcers and liver graft rejection, in which reduced EOLA1 expression correlates with exacerbated inflammatory responses and tissue injury."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "8"}]}, {"type": "t", "text": " Finally, structural analysis indicates that EOLA1 adopts a β‑barrel fold similar to an activating signal cointegrator‑1 homology (ASCH) domain, suggesting a conserved mechanism for intermolecular interactions that may underlie its regulatory functions."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "10"}]}, {"type": "t", "text": " Collectively, these studies support a model in which EOLA1 serves as a critical intracellular modulator that fine‑tunes inflammatory signaling and promotes endothelial cell survival and proliferation."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "11"}]}, {"type": "t", "text": "\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Yueming Liu, Hairong Liu, Wenhai Chen, et al. 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