{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n EVI2B is a gene located within intron 27b of the NF1 locus that encodes a transmembrane glycoprotein—designated CD361—with an N‐terminal signal peptide, an extracellular domain harboring potential glycosylation sites, a single hydrophobic transmembrane domain, and a cytoplasmic region (also described for its homolog EVI2A)."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": " In hematopoietic cells, EVI2B is a direct transcriptional target of the master regulator C/EBPα and is abundantly expressed on the surface of primary cells, reaching highest levels in mature granulocytes; its expression is crucial for normal myeloid differentiation and the functionality of hematopoietic progenitors, as evidenced by impaired lineage development and colony formation upon its downregulation."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": " In addition, modulation of EVI2B expression has been observed in contexts beyond hematopoiesis—for example, its downregulation by agouti signaling protein in melanoma cells suggests a potential role in melanogenesis."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n \n "}, {"type": "p", "children": [{"type": "t", "text": "\n Beyond its developmental role, EVI2B serves as a valuable biomarker in various pathophysiological contexts. Its cell surface expression has been utilized in immunophenotyping studies, including those involving B cells and plasmacytoid dendritic cells"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": ", and clusters of genes that include EVI2B have been associated with unfavorable outcomes in hematological malignancies such as chronic lymphocytic leukemia."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "5"}]}, {"type": "t", "text": " In solid tumors, dysregulated EVI2B expression appears to confer tumor suppressor properties; in colorectal cancer, for instance, EVI2B is among the genes whose altered levels correlate with reduced proliferation, migration, and invasion, underscoring its prognostic significance."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "6"}]}, {"type": "t", "text": "\n "}]}, {"type": "t", "text": "\n \n "}, {"type": "p", "children": [{"type": "t", "text": "\n Collectively, the evidence supports a multifaceted role for EVI2B in both normal and malignant contexts: it contributes to myeloid differentiation and hematopoietic progenitor function, serves as a surface marker for immune cells, and may exert tumor suppressive effects in certain cancers. Although the molecular mechanisms underlying its regulatory functions remain to be fully elucidated, current findings highlight EVI2B as a promising biomarker and potential therapeutic target across a broad spectrum of disorders.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "R M Cawthon, L B Andersen, A M Buchberg, et al. 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