{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nThe discovery of novel peptide hormones is often complicated by the need for specific posttranslational processing, and one innovative approach to overcome this challenge involves engineering endocrine host cells that are competent for such modifications. In this context, candidate preprohormones, initially selected by bioinformatics, were expressed in these cells—demonstrated by successful processing of established hormones such as insulin and glucagon—and used to screen G-protein-coupled receptors. This strategy led to the identification of FAM237A as a specific activator of GPR83, a receptor associated with functions in the central nervous system and regulatory T-cell activity."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n \nDis primis duis mauris potenti non at sociosqu ultricies massa. Tincidunt vel sagittis quam convallis varius phasellus tincidunt. Libero ultrices libero fermentum lectus ex nunc felis. In the case of FAM237A, the biologically active form was determined to be a C-terminally cleaved, amidated 9 kDa secreted protein. Additionally, a homologous protein, FAM237B, which shares 64% sequence identity with FAM237A, was shown to undergo similar posttranslational modifications and activate GPR83, albeit with reduced potency."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n \nFusce class lectus fringilla commodo sit blandit pellentesque. Mattis sem magna metus luctus purus vivamus adipiscing ex. These findings not only highlight the functional role of FAM237A as a novel peptide hormone modulating GPR83 activity, but they also validate the utility of the employed screening approach for uncovering other hormones that depend on specific processing events for activation."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Nathan A Sallee, Ernestine Lee, Atossa Leffert, et al. "}, {"type": "b", "children": [{"type": "t", "text": "A Pilot Screen of a Novel Peptide Hormone Library Identified Candidate GPR83 Ligands."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "SLAS Discov (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1177/2472555220934807"}], "href": "https://doi.org/10.1177/2472555220934807"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "32713278"}], "href": "https://pubmed.ncbi.nlm.nih.gov/32713278"}]}]}]}