{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n HTR2A‐AS1 is an antisense long noncoding RNA arising from the HTR2A gene locus whose structure and expression may have functional implications in both the nervous system and liver cancer. In brain tissue, RNA‐sequencing studies have redefined HTR2A‐AS1 to span approximately 474 kilobases with 18 exons—substantially more than previously annotated—and revealed that several of its exons overlap with those of the sense HTR2A transcript. These findings raise the possibility of complementary base‐pairing or regulatory interactions between the overlapping regions, with common single nucleotide polymorphisms (such as rs6311) modulating the expression of both the sense and antisense transcripts."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n \n "}, {"type": "p", "children": [{"type": "t", "text": "\n In addition to its potential regulatory role in the central nervous system, HTR2A‐AS1 has emerged as a prognostic marker in hepatocellular carcinoma (HCC). Bioinformatic analyses of HCC transcriptomes have identified HTR2A‐AS1 as part of a competing endogenous RNA signature that accurately distinguishes high‐risk from low‐risk patients, suggesting that its expression is closely linked to hepatocarcinogenesis."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": " \n "}]}, {"type": "t", "text": "\n \n "}, {"type": "p", "children": [{"type": "t", "text": "\n Functional studies in HCC cell models further support a tumor‐suppressive role for HTR2A‐AS1. Treatment with Patrinia herba aqueous extract was found to upregulate HTR2A‐AS1 expression, which in turn inhibited cell proliferation, colony formation, invasion, and migration while promoting apoptosis. Overexpression of HTR2A‐AS1 mimicked these effects, whereas its inhibition mitigated them, suggesting that HTR2A‐AS1 may mediate part of the extract’s anti-cancer activity in hepatocellular carcinoma cells."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": "\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Cara L Ruble, Ryan M Smith, John Calley, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Genomic structure and expression of the human serotonin 2A receptor gene (HTR2A) locus: identification of novel HTR2A and antisense (HTR2A-AS1) exons."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "BMC Genet (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1186/s12863-015-0325-6"}], "href": "https://doi.org/10.1186/s12863-015-0325-6"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26738766"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26738766"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Jian-Rong Sun, Chen-Fan Kong, Kun-Min Xiao, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Integrated Analysis of lncRNA-Mediated ceRNA Network Reveals a Prognostic Signature for Hepatocellular Carcinoma."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Front Genet (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3389/fgene.2020.602542"}], "href": "https://doi.org/10.3389/fgene.2020.602542"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "33381151"}], "href": "https://pubmed.ncbi.nlm.nih.gov/33381151"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Zhenhua Guan, Jing Chen, Xueliang Li "}, {"type": "b", "children": [{"type": "t", "text": "Patrinia herba aqueous extract on the proliferation, apoptosis, invasion and migration of hepatocellular carcinoma cells."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell Mol Biol (Noisy-le-grand) (2020)"}]}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "32538757"}], "href": "https://pubmed.ncbi.nlm.nih.gov/32538757"}]}]}]}