{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n Although the study in"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "does not explicitly mention HTR5A‐AS1, its emphasis on differential transcript regulation in intestinal M cells versus Caco‑2 cells provides a context for understanding potential roles of long noncoding RNAs—including antisense transcripts such as HTR5A‑AS1—in modulating immune responses. In this investigation, distinct expression patterns were observed when comparing uninfected M cells with epithelial Caco‑2 cells, as well as during infection with wild‐type and ΔspeG mutant Salmonella Typhimurium. The data reveal that infection elicits a reprogramming of the transcriptome, with some transcripts (including various long noncoding RNAs) undergoing significant up‐ or down‐regulation in a speG‑dependent or ‑independent manner (1). Although HTR5A‑AS1 was not specifically analyzed, by analogy with other long noncoding RNAs highlighted in the study, one could hypothesize that HTR5A‑AS1 may contribute to the regulatory networks that govern M cell functional identity and immune modulation in response to bacterial challenge. Further research would be needed to characterize its precise mechanistic contribution, especially regarding its potential influence on cellular differentiation, pathogen response, and the broader orchestration of mucosal immunity.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Ke-Chuan Wang, Chih-Hung Huang, Ching-Jou Huang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Impacts of Salmonella enterica Serovar Typhimurium and Its speG Gene on the Transcriptomes of In Vitro M Cells and Caco-2 Cells."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS One (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pone.0153444"}], "href": "https://doi.org/10.1371/journal.pone.0153444"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "27064787"}], "href": "https://pubmed.ncbi.nlm.nih.gov/27064787"}]}]}]}