{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n INE2, the product of the inebriated (ine) gene in Drosophila, has been shown to play important roles in both neuronal signaling and systemic water homeostasis. In the nervous system, INE2 functions as a putative neurotransmitter transporter that appears to facilitate the clearance and recycling of the histamine metabolite carcinine at the photoreceptor synapse. Genetic and pharmacological evidence indicate that loss‐of‐function mutations in ine lead to aberrant electroretinograph (ERG) responses, supporting its role in maintaining proper histaminergic neurotransmission by preventing carcinine accumulation."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": " In addition, INE2 is expressed in the basolateral membrane of anterior hindgut epithelial cells, where it is essential for water conservation and absorption. Under conditions of external hypertonicity or desiccation, the absence of functional INE2 results in severe dehydration due to a failure in proper water homeostasis, a defect that is fully rescued by targeted expression of ine in the hindgut."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": " Together, these findings demonstrate that INE2 serves a dual function in Drosophila by both regulating neurotransmitter dynamics in the visual system and maintaining systemic water balance.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Brendan A Gavin, Susan E Arruda, Patrick J Dolph "}, {"type": "b", "children": [{"type": "t", "text": "The role of carcinine in signaling at the Drosophila photoreceptor synapse."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS Genet (2007)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pgen.0030206"}], "href": "https://doi.org/10.1371/journal.pgen.0030206"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "18069895"}], "href": "https://pubmed.ncbi.nlm.nih.gov/18069895"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Zhuo Luan, Caitlin Quigley, Hong-Sheng Li "}, {"type": "b", "children": [{"type": "t", "text": "The putative Na⁺/Cl⁻-dependent neurotransmitter/osmolyte transporter inebriated in the Drosophila hindgut is essential for the maintenance of systemic water homeostasis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Sci Rep (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/srep07993"}], "href": "https://doi.org/10.1038/srep07993"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "25613130"}], "href": "https://pubmed.ncbi.nlm.nih.gov/25613130"}]}]}]}