{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n In one study focused on establishing reliable endogenous control genes for relative quantification in colorectal cancer tissues"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ", a panel of thirteen candidate genes was evaluated. Among these candidates, KRTAP12‑3 was assessed for its potential as a normalization control in RQ‑PCR data analysis. Although KRTAP12‑3 was included in the panel, its expression stability in colorectal tissue did not prove optimal when compared to the two most robust candidates identified, B2M and PPIA. This finding underscores the importance of rigorously testing candidate control genes to ensure accurate gene expression measurements, which are critical for reliable cancer diagnosis, prognosis, and treatment planning (1)."}]}, {"type": "t", "text": "\n \n "}, {"type": "p", "children": [{"type": "t", "text": "\n Complementary studies have expanded our understanding of the molecular underpinnings of human disease by exploring ethnicity‑specific SNPs and their links to complex clinical traits"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": ", delineating the protein–protein interaction networks within ciliary organelles and their potential roles in disorders such as 3M syndrome"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": ", and identifying novel drivers of epithelial‑to‑mesenchymal transition that may contribute to cancer metastasis and therapeutic resistance."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": " Together, these investigations highlight the multifaceted approaches being used to dissect the complexity of gene regulation, cellular structures, and pathological processes.\n "}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Elrasheid A H Kheirelseid, Kah Hoong Chang, John Newell, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Identification of endogenous control genes for normalisation of real-time quantitative PCR data in colorectal cancer."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "BMC Mol Biol (2010)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1186/1471-2199-11-12"}], "href": "https://doi.org/10.1186/1471-2199-11-12"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "20122155"}], "href": "https://pubmed.ncbi.nlm.nih.gov/20122155"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Hyoyoung Kim, Won Gi Yoo, Junhyung Park, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Semantic Modeling for SNPs Associated with Ethnic Disparities in HapMap Samples."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Genomics Inform (2014)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.5808/GI.2014.12.1.35"}], "href": "https://doi.org/10.5808/GI.2014.12.1.35"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "24748859"}], "href": "https://pubmed.ncbi.nlm.nih.gov/24748859"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Karsten Boldt, Jeroen van Reeuwijk, Qianhao Lu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "An organelle-specific protein landscape identifies novel diseases and molecular mechanisms."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Nat Commun (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/ncomms11491"}], "href": "https://doi.org/10.1038/ncomms11491"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "27173435"}], "href": "https://pubmed.ncbi.nlm.nih.gov/27173435"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Dubravka Škalamera, Mareike Dahmer-Heath, Alexander J Stevenson, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Genome-wide gain-of-function screen for genes that induce epithelial-to-mesenchymal transition in breast cancer."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Oncotarget (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.18632/oncotarget.11314"}], "href": "https://doi.org/10.18632/oncotarget.11314"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "27876705"}], "href": "https://pubmed.ncbi.nlm.nih.gov/27876705"}]}]}]}