{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\n Recent studies have highlighted the importance of non‐coding RNA regulation in diverse biological contexts, including cancer stem cell biology and spermatogenesis. In an investigation of esophageal squamous cell carcinoma stem‐like cells, genome‐wide analyses revealed distinct DNA methylation profiles compared to non‐stem cells; however, the direct involvement of long non‐coding RNAs (lncRNAs) was not specifically addressed in that study (1). In contrast, transcriptomic studies in human germ cells have consistently identified a set of lncRNAs—including LINC00658—as being differentially expressed during spermatogenesis. The consistent deregulation of LINC00658 across independent studies, along with its predicted interactions with targets such as cell cycle regulators (e.g., CDKN family members), epigenetic modifiers (EZH2, SUZ12) and other genes implicated in cellular differentiation and proliferation, suggests that it may play a regulatory role in germ cell development (2). Collectively, while the methylation study of esophageal cancer stem‐like cells underscores the complexity of epigenetic regulation in stem cell populations, the identification of LINC00658 in studies of spermatogenesis points to its potential function as a modulator of gene expression networks that govern cellular proliferation and differentiation. Further research may elucidate whether similar mechanisms underlie regulatory processes in both cancer stem cells and germ cells.\n "}]}, {"type": "t", "text": "\n "}, {"type": "p", "children": [{"type": "t", "text": "\n References:."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Xiying Yu, Ying Teng, Xingran Jiang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Genome-Wide DNA Methylation Pattern of Cancer Stem Cells in Esophageal Cancer."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Technol Cancer Res Treat (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1177/1533033820983793"}], "href": "https://doi.org/10.1177/1533033820983793"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "33357046"}], "href": "https://pubmed.ncbi.nlm.nih.gov/33357046"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Meghali Joshi, Singh Rajender "}, {"type": "b", "children": [{"type": "t", "text": "Long non-coding RNAs (lncRNAs) in spermatogenesis and male infertility."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Reprod Biol Endocrinol (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1186/s12958-020-00660-6"}], "href": "https://doi.org/10.1186/s12958-020-00660-6"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "33126901"}], "href": "https://pubmed.ncbi.nlm.nih.gov/33126901"}]}]}]}