{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nIn acute myeloid leukemia (AML) cells, the long non‐coding RNA C9ORF139 (also referred to as LINC02908 in some contexts) functions as an oncogenic regulator. It acts by “sponging” microRNA miR‐24-3p, thereby modulating the expression of TAOK1. By binding miR‐24-3p, C9ORF139 prevents miR‐24-3p from inhibiting TAOK1, leading to increased TAOK1 expression. This interaction was confirmed by luciferase reporter assays showing that the presence of an intact binding site in C9ORF139 is critical for miR‐24-3p regulation."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nFunctional studies in AML cell lines (HL-60 and THP-1) revealed that knockdown of C9ORF139 significantly reduces cell proliferation, migration, and invasion while increasing apoptosis. Rescue experiments demonstrated that inhibition of miR‐24-3p or overexpression of TAOK1 could partially reverse these effects, reinforcing the pathway’s importance in AML cellular dynamics. In addition, the decrease in phosphorylated Raf, MEK, and ERK levels following C9ORF139 knockdown suggests that the oncogenic activity of this lncRNA is mediated at least in part through the Raf/MEK/ERK signaling cascade."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nIn vivo experiments further confirmed the oncogenic role of C9ORF139. In a nude mouse model, suppression of C9ORF139 expression resulted in significantly smaller and lighter subcutaneous tumors compared to controls. Overall, these findings highlight that C9ORF139/LINC02908 drives AML progression by regulating the miR‐24-3p/TAOK1 axis and activating downstream proliferative and survival signaling pathways."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "W Qin, X H Cai, W M Han, et al. "}, {"type": "b", "children": [{"type": "t", "text": "[The role and mechanism of lncRNA C9ORF139 targeting miR-24-3P/TAOK1 in regulating the proliferation of acute myeloid leukemia cells]."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Zhonghua Yi Xue Za Zhi (2022)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3760/cma.j.cn112137-20210703-01501"}], "href": "https://doi.org/10.3760/cma.j.cn112137-20210703-01501"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "35196780"}], "href": "https://pubmed.ncbi.nlm.nih.gov/35196780"}]}]}]}