{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nRecent studies have demonstrated that MAFIP acts as a potent tumor suppressor by modulating the NF-κB signaling pathway in cancer cells. In cervical cancer, MAFIP is downregulated in cervical intraepithelial neoplasia tissues, and its overexpression in HeLa cells blocks tumorigenesis in nude mice. Mechanistically, MAFIP impedes the NF-κB pathway by inhibiting the phosphorylation of IKK and IκBα, preventing IκBα degradation and the subsequent nuclear translocation of p65, which in turn suppresses c-myc induction. Conversely, reducing MAFIP levels activates NF-κB signaling and enhances cell proliferation, underscoring its role as a tumor suppressor in cervical cancer."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "In parallel, investigation into MafF interacting protein (MIP) has revealed its inhibitory effects on cancer cell proliferation, especially in liver cancer. MIP suppresses colony formation in liver-derived SMMC7721 cells (with a noted, albeit weaker, effect in HeLa cells) and exerts its function, at least in part, by directly binding to the NF-κB essential modulator (NEMO). The interaction between MIP and NEMO, confirmed by yeast two-hybrid screening, GST pull-down, and co-immunoprecipitation assays, links MIP to the regulation of NF-κB signaling, thereby contributing to its anti-proliferative effects."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Yang Li, Yao Yu, Yan Zhang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "MAFIP is a tumor suppressor in cervical cancer that inhibits activation of the nuclear factor-kappa B pathway."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cancer Sci (2011)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1111/j.1349-7006.2011.02061.x"}], "href": "https://doi.org/10.1111/j.1349-7006.2011.02061.x"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21834855"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21834855"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Xiaoxia Ye, Hong Lu, Keke Huo, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Finding a novel interacting protein of the hepatic carcinoma related gene MIP: NF-κB essential modulator (NEMO)."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Oncol Rep (2011)"}]}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "21109981"}], "href": "https://pubmed.ncbi.nlm.nih.gov/21109981"}]}]}]}