{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nFAM188B, also known as MINDY4, is an evolutionarily conserved gene whose mRNA is highly expressed in a variety of solid tumors, including colorectal cancer. Differential exon usage in cancers initially hinted at its potential importance in tumor biology, prompting further investigation into its function."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nFunctional studies employing siRNA‐mediated knockdown of FAM188B/MINDY4 in colon cancer cell lines have demonstrated that reduced expression of this gene results in pronounced cell growth inhibition, primarily due to the induction of apoptosis. This knockdown significantly increases p53 levels and its activation, thereby upregulating p53‐dependent pro‐apoptotic proteins such as PUMA and BAX. Proteomic analyses further revealed that FAM188B interacts with p53 and the deubiquitinating enzyme USP7, where disruption of the USP7-binding motif in FAM188B diminishes complex formation and leads to decreased ubiquitination of p53."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nCollectively, these findings suggest that MINDY4 functions as a putative oncogene by modulating the stability of p53 through its interaction with USP7, thereby controlling pathways that affect cell survival and tumor growth. Inhibition of MINDY4 not only decreases colony formation and anchorage-independent growth in vitro but also reduces tumor growth in xenografted mice, making it a promising target for therapeutic intervention."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Eun-Seok Choi, Hanna Lee, Jee Young Sung, et al. "}, {"type": "b", "children": [{"type": "t", "text": "FAM188B enhances cell survival via interaction with USP7."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell Death Dis (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41419-018-0650-6"}], "href": "https://doi.org/10.1038/s41419-018-0650-6"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "29795372"}], "href": "https://pubmed.ncbi.nlm.nih.gov/29795372"}]}]}]}