{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nRecent findings have indicated that miR‑323b, particularly the hsa‑miR‑323b‑5p isoform, may serve as an effective circulating biomarker. In type 2 diabetic patients with critical limb ischemia (CLI), this miRNA is markedly up‑regulated, with serum levels demonstrating promising diagnostic accuracy in distinguishing CLI from non‑CLI conditions in these patients. Its potential utility as a non‑invasive biomarker underlines its clinical relevance in assessing vascular complications associated with diabetes."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nBeyond its diagnostic potential in vascular disease, genetic variants within the precursor of miR‑323b have been implicated in modulating host–virus interactions and cancer risk. A prevalent single nucleotide polymorphism (rs56103835C>T) in the pre‑miRNA region has been associated with enhanced hepatitis B virus (HBV) replication, suggesting that the wild‑type miR‑323b can suppress viral DNA levels, whereas its variant may predispose individuals to persistent infection. Similarly, investigations in breast cancer populations are examining this same variant for its association with tumor development, supporting a potential tumor suppressor role for miR‑323b in this context."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nIn contrast to its tumor suppressive indications in certain contexts, miR‑323‑3p appears to function as an oncogene in non‑small‑cell lung cancer (NSCLC). Elevated levels of miR‑323‑3p in lung cancer cell lines contribute to enhanced cell proliferation by directly targeting and suppressing TMEFF2 expression. This down‑regulation of TMEFF2 in turn hinders the activation of key signaling cascades, including the AKT and ERK pathways, thereby promoting tumorigenesis and cellular progression in NSCLC."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Biao Cheng, Ju-Yi Li, Xiao-Chao Li, et al. "}, {"type": "b", "children": [{"type": "t", "text": "MiR-323b-5p acts as a novel diagnostic biomarker for critical limb ischemia in type 2 diabetic patients."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Sci Rep (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41598-018-33310-4"}], "href": "https://doi.org/10.1038/s41598-018-33310-4"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30305681"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30305681"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "S J Yu, J-W Kim, J-H Lee, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Association of a microRNA-323b polymorphism with the persistence of hepatitis B virus infection by the enhancement of viral replication."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Viral Hepat (2014)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1111/jvh.12215"}], "href": "https://doi.org/10.1111/jvh.12215"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "24341744"}], "href": "https://pubmed.ncbi.nlm.nih.gov/24341744"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Negin Naderi, Maryam Peymani, Kamran Ghaedi "}, {"type": "b", "children": [{"type": "t", "text": "The protective role of rs56103835 against breast cancer onset in the Iranian population."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Genet Genomic Med (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1002/mgg3.540"}], "href": "https://doi.org/10.1002/mgg3.540"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30706682"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30706682"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Ji-Min Fan, Zheng-Rong Zheng, Yi-Ming Zeng, et al. "}, {"type": "b", "children": [{"type": "t", "text": "MiR-323-3p Targeting Transmembrane Protein with EGF-Like and 2 Follistatin Domain (TMEFF2) Inhibits Human Lung Cancer A549 Cell Apoptosis by Regulation of AKT and ERK Signaling Pathways."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Med Sci Monit (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.12659/MSM.919454"}], "href": "https://doi.org/10.12659/MSM.919454"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "32009129"}], "href": "https://pubmed.ncbi.nlm.nih.gov/32009129"}]}]}]}