{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nIn colorectal cancer, miR-3622a-3p is markedly downregulated relative to normal tissues, and its reduced expression is associated with enhanced tumor malignancy. Functional studies demonstrated that restoration of miR-3622a-3p levels suppresses proliferation, invasion, and stemness properties by directly targeting and degrading SALL4 mRNA, thereby inhibiting epithelial–mesenchymal transition and promoting apoptosis. These effects were confirmed in various in vitro assays as well as in vivo tumor xenograft and organoid models, underscoring its tumor‐suppressive role in colorectal cancer."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "Conversely, in bladder cancer, miR-3622a acts as an oncogenic microRNA. Here, miR-3622a expression is negatively correlated with overall patient survival and functions to potentiate malignant behavior by downregulating the metastasis suppressor gene LASS2. Overexpression of miR-3622a in bladder cancer cells leads to increased proliferation and invasion, highlighting its role in promoting tumor progression in this context."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "Collectively, these studies reveal that the function of miR-3622a is highly context-dependent: while it serves as a tumor suppressor in colorectal cancer via SALL4 inhibition, it concurrently acts as a tumor promoter in bladder cancer through attenuation of LASS2. These insights into the dualistic nature of miR-3622a underscore the complexity of microRNA regulation in cancer and may provide novel avenues for tailored therapeutic strategies."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Shuchen Chang, Guangli Sun, Dan Zhang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "MiR-3622a-3p acts as a tumor suppressor in colorectal cancer by reducing stemness features and EMT through targeting spalt-like transcription factor 4."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell Death Dis (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41419-020-02789-z"}], "href": "https://doi.org/10.1038/s41419-020-02789-z"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "32719361"}], "href": "https://pubmed.ncbi.nlm.nih.gov/32719361"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Shi Fu, Ting Luan, Changyi Jiang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "miR-3622a promotes proliferation and invasion of bladder cancer cells by downregulating LASS2."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Gene (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.gene.2019.02.083"}], "href": "https://doi.org/10.1016/j.gene.2019.02.083"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30898713"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30898713"}]}]}]}