{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nRecent studies have underscored the diversity of molecular investigations in biomedical research. For example, one study employed a QM/MM model to elucidate structure–function relationships underlying olfactory signal transduction in a mouse olfactory receptor (MOR244-3), demonstrating how detailed computational models can advance our understanding of receptor–ligand interactions (a study that did not involve MIR3650)."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n \nIn hepatocellular carcinoma (HCC), MIR3650 has emerged as an important tumor‐suppressive microRNA. Investigators found that its expression was frequently decreased in HCC tissues, with lower levels correlating significantly with tumor metastasis and poorer patient survival. Functional experiments revealed that forced expression of MIR3650 inhibited cell migration and the epithelial–mesenchymal transition (EMT), a key process in cancer progression, and mechanistic studies identified neurofascin (NFASC) as a direct target of MIR3650. Consequently, MIR3650 appears to repress HCC progression by downregulating NFASC expression."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "\n \nAlthough MIR3650’s tumor‐suppressive role is evident in HCC, its function in other cancers remains to be fully elucidated. In gastric cancer, where microRNAs also control diverse regulatory networks, the specific role and mechanistic impact of MIR3650 are not yet well understood. Clarifying its function in this context could further reveal the broader significance of MIR3650 in cancer biology and potentially expand its utility as a prognostic marker or therapeutic target."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Sivakumar Sekharan, Mehmed Z Ertem, Hanyi Zhuang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "QM/MM model of the mouse olfactory receptor MOR244-3 validated by site-directed mutagenesis experiments."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biophys J (2014)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.bpj.2014.07.031"}], "href": "https://doi.org/10.1016/j.bpj.2014.07.031"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "25185561"}], "href": "https://pubmed.ncbi.nlm.nih.gov/25185561"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Jian Wu, Wen-Jin Huang, Hong-Li Xi, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Tumor-suppressive miR-3650 inhibits tumor metastasis by directly targeting NFASC in hepatocellular carcinoma."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Aging (Albany NY) (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.18632/aging.101981"}], "href": "https://doi.org/10.18632/aging.101981"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "31163018"}], "href": "https://pubmed.ncbi.nlm.nih.gov/31163018"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Xiansheng Yang, Juncai Wen, Qingjun He, et al. "}, {"type": "b", "children": [{"type": "t", "text": "MicroRNA3650 Promotes Gastric Cancer Proliferation and Migration through the PTEN/PI3K-AKT-mTOR and Hippo Pathways."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Protein Pept Lett (2023)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.2174/0109298665265642231020043809"}], "href": "https://doi.org/10.2174/0109298665265642231020043809"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "38031771"}], "href": "https://pubmed.ncbi.nlm.nih.gov/38031771"}]}]}]}