{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nMIR3689A is an emerging microRNA implicated in the regulation of gene expression events that contribute to cellular drug responses. In hepatocellular carcinoma (HCC), recent genomic analyses have identified MIR3689A as a key modulator of sorafenib resistance, suggesting that its altered expression may influence signaling pathways involved in cellular survival and response to therapy. This discovery positions MIR3689A as a potential biomarker and therapeutic target for overcoming resistance in HCC."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nAlthough the study focused on mitochondrial metabolic regulation via METTL20 did not directly examine MIR3689A, it underscores the importance of metabolic adaptation in cellular stress responses. In that work, METTL20 was shown to methylate mitochondrial components—a modification that enhanced β‐oxidation and thermogenesis, thereby affecting energy production under metabolically demanding conditions. While MIR3689A was not implicated in these mitochondrial processes, the interplay of key regulatory factors in metabolism and drug resistance highlights a broader biological context in which microRNAs may fine‐tune responses to both metabolic challenges and anticancer treatments."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nCollectively, these observations suggest that MIR3689A plays a crucial role in modulating therapeutic outcomes in HCC by contributing to sorafenib resistance. Furthermore, the metabolic insights gained from studies on mitochondrial regulators like METTL20 offer an intriguing framework for future investigations to determine whether MIR3689A, beyond its function in drug resistance, might also intersect with metabolic pathways. Such integrative studies could ultimately inform strategies to enhance treatment efficacy by targeting microRNA‐mediated networks."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Yuanjun Lu, Yau-Tuen Chan, Junyu Wu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "CRISPR/Cas9 screens unravel miR-3689a-3p regulating sorafenib resistance in hepatocellular carcinoma via suppressing CCS/SOD1-dependent mitochondrial oxidative stress."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Drug Resist Updat (2023)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.drup.2023.101015"}], "href": "https://doi.org/10.1016/j.drup.2023.101015"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "37924725"}], "href": "https://pubmed.ncbi.nlm.nih.gov/37924725"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Tadahiro Shimazu, Tamio Furuse, Shabeesh Balan, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Role of METTL20 in regulating β-oxidation and heat production in mice under fasting or ketogenic conditions."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Sci Rep (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41598-018-19615-4"}], "href": "https://doi.org/10.1038/s41598-018-19615-4"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "29352221"}], "href": "https://pubmed.ncbi.nlm.nih.gov/29352221"}]}]}]}