{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nmiR‑412, particularly its 5p isoform, has been implicated in the regulation of vascular endothelial cell function in the context of hemorrhoid pathogenesis. In hemorrhoid tissues, miR‑412-5p expression is markedly decreased relative to normal tissue, a change that relieves its inhibitory control over target mRNAs. Experimental data indicate that miR‑412-5p directly binds to the 3′‑UTR of Xpo1 mRNA, resulting in its silencing and subsequent modulation of downstream signaling. With Xpo1 repressed, the tumor suppressor protein p53 is retained within the nucleus where it is able to promote the expression of cell cycle regulators such as p66SHC and p16. Consequently, the overexpression of miR‑412-5p in endothelial cells leads to reduced proliferation, migration, and vascular structure formation, thereby impeding angiogenesis. In contrast, the downregulation of miR‑412-5p observed in hemorrhoid tissues facilitates increased Xpo1 expression and disrupts p53 nuclear function, ultimately contributing to unchecked vascular endothelial cell division and angiogenesis."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Chen Wang, Hong Lu, Chunsheng Luo, et al. "}, {"type": "b", "children": [{"type": "t", "text": "miR-412-5p targets Xpo1 to regulate angiogenesis in hemorrhoid tissue."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Gene (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.gene.2019.04.058"}], "href": "https://doi.org/10.1016/j.gene.2019.04.058"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "31026569"}], "href": "https://pubmed.ncbi.nlm.nih.gov/31026569"}]}]}]}