{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nRecent studies have highlighted the potential of novel microRNAs as non-invasive biomarkers in cancer detection. In one investigation, miR-4316 was examined in the context of colorectal cancer screening via liquid biopsy, aiming to offer a less invasive alternative to colonoscopy. Although its precise mechanistic role in colorectal tumor biology was not fully delineated, the altered expression of miR-4316 suggests its potential as an early detection marker in colorectal cancer."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n\nIn breast cancer, miR-4316 has emerged as a central component of a competing endogenous RNA regulatory network. Here, the circular RNA circMYO9B acts as a molecular sponge, binding miR-4316 and thereby relieving its repression on the FOXP4 gene. The subsequent upregulation of FOXP4 was shown to promote cellular proliferation, migration, and invasion, which, in turn, correlated with a worse prognosis for patients."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "\n\nSimilarly, in bladder cancer, miR-4316 is functionally intertwined with the long noncoding RNA CALML3-AS1. CALML3-AS1 directly interacts with miR-4316, decreasing its availability and leading to the derepression of the downstream target ZBTB2. Elevated ZBTB2 expression contributes to tumorigenesis by repressing the cell-cycle inhibitor p21 and enhancing the transcription of PDK4, thereby facilitating cancer progression."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Paweł Krawczyk, Tomasz Powrózek, Tomasz Olesiński, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Evaluation of miR-506 and miR-4316 expression in early and non-invasive diagnosis of colorectal cancer."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Int J Colorectal Dis (2017)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1007/s00384-017-2814-8"}], "href": "https://doi.org/10.1007/s00384-017-2814-8"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "28405738"}], "href": "https://pubmed.ncbi.nlm.nih.gov/28405738"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Nan Wang, Yuanting Gu, Lin Li, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Circular RNA circMYO9B facilitates breast cancer cell proliferation and invasiveness via upregulating FOXP4 expression by sponging miR-4316."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Arch Biochem Biophys (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.abb.2018.04.017"}], "href": "https://doi.org/10.1016/j.abb.2018.04.017"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "29702064"}], "href": "https://pubmed.ncbi.nlm.nih.gov/29702064"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Feng Wang, Yanwen Zu, Weiping Huang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "LncRNA CALML3-AS1 promotes tumorigenesis of bladder cancer via regulating ZBTB2 by suppression of microRNA-4316."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biochem Biophys Res Commun (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.bbrc.2018.08.150"}], "href": "https://doi.org/10.1016/j.bbrc.2018.08.150"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30177388"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30177388"}]}]}]}