{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nRecent studies indicate that miR-4474 plays a pivotal role in the progression of Fusobacterium nucleatum-induced colorectal cancer (CRC). Investigations employing miRNA microarray analyses and RT-PCR revealed that miR-4474 is significantly upregulated in CRC tissues infected with F. nucleatum, while its target gene, CREBBP—a key regulator in cancer-related signaling pathways—is concurrently downregulated. Bioinformatic analyses and experimental validation identified CREBBP as a direct target of miR-4474, suggesting that the miRNA contributes to tumor progression through posttranscriptional suppression of a gene critical for maintaining normal cell signaling and growth."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nIn contrast, investigations into gene expression within the central nervous system, such as studies examining olfactory receptor profiles in both normal and Alzheimer’s disease-like brains, have not implicated miR-4474 in neural regulatory processes. These findings underscore the highly context-specific functions of miRNAs, where miR-4474 appears to have a pronounced role in modulating cancer-associated pathways in the colorectal epithelium, while remaining functionally unassociated with the pathways examined in neural tissues."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Yu-Yang Feng, Dong-Zhu Zeng, Ya-Nan Tong, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Alteration of microRNA-4474/4717 expression and CREB-binding protein in human colorectal cancer tissues infected with Fusobacterium nucleatum."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS One (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pone.0215088"}], "href": "https://doi.org/10.1371/journal.pone.0215088"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30951563"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30951563"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Fanny Gaudel, Delphine Stephan, Véréna Landel, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Expression of the Cerebral Olfactory Receptors Olfr110/111 and Olfr544 Is Altered During Aging and in Alzheimer's Disease-Like Mice."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Neurobiol (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1007/s12035-018-1196-4"}], "href": "https://doi.org/10.1007/s12035-018-1196-4"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "29984401"}], "href": "https://pubmed.ncbi.nlm.nih.gov/29984401"}]}]}]}