{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nRecent studies have begun to implicate MIR4483 as a regulatory microRNA in pathways that intersect with those governed by proteins involved in renal ciliopathy and vascular remodeling. For example, in murine inner medullary collecting duct cells, depletion of ANKS3 triggered widespread metabolic disturbances—including disruptions in amino acid, purine/pyrimidine, and tryptophan-niacin pathways—and activated DNA damage responses. Although MIR4483 is not directly mentioned in that study, such metabolic and repair pathways are typical targets of microRNA regulation, suggesting that MIR4483 may modulate similar networks in kidney cells."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nFurther insights into the cellular roles of ANKS3 come from work demonstrating its function in protein interaction networks that control cell cycle progression and intracellular localization signals. Studies showed that ANKS3 interacts with kinases such as Nek7, where its modification mediates the retention of Nek7 in the cytoplasm, potentially affecting downstream signaling involved in apoptosis and proliferation. Given the common microRNA involvement in fine-tuning such signaling cascades, MIR4483 might similarly act to adjust these protein–protein interaction networks, thereby influencing cellular processes that are vital for maintaining tissue architecture and function."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nExpanding the functional perspective beyond the kidney, research into nephronophthisis and polycystic kidney disease emphasized the importance of ANKS3–ANKS6 interactions in ciliogenesis, transcriptional regulation of vasopressin-induced genes, and pathways controlling apoptosis and cell proliferation. In parallel, an independent study aiming to elucidate the role of microRNAs in cerebral aneurysm formation raises the possibility that MIR4483 may contribute to vascular remodeling and endothelial integrity by targeting transcripts involved in ciliary structure and fluid balance. Together, these investigations indicate that MIR4483 could serve as an integrative modulator—linking metabolic homeostasis, DNA damage responses, and key cellular signaling cascades in both renal and vascular tissues."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Manuel Schlimpert, Simon Lagies, Vadym Budnyk, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Metabolic Phenotyping of Anks3 Depletion in mIMCD-3 cells - a Putative Nephronophthisis Candidate."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Sci Rep (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41598-018-27389-y"}], "href": "https://doi.org/10.1038/s41598-018-27389-y"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "29899363"}], "href": "https://pubmed.ncbi.nlm.nih.gov/29899363"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Haribaskar Ramachandran, Christina Engel, Barbara Müller, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Anks3 alters the sub-cellular localization of the Nek7 kinase."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biochem Biophys Res Commun (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.bbrc.2015.07.063"}], "href": "https://doi.org/10.1016/j.bbrc.2015.07.063"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26188091"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26188091"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Laure Delestré, Zeineb Bakey, Cécilia Prado, et al. "}, {"type": "b", "children": [{"type": "t", "text": "ANKS3 Co-Localises with ANKS6 in Mouse Renal Cilia and Is Associated with Vasopressin Signaling and Apoptosis In Vivo in Mice."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS One (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pone.0136781"}], "href": "https://doi.org/10.1371/journal.pone.0136781"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26327442"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26327442"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Zeki Boga, Ozlem Anlas, Vedat Acik, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The Role of miR-26a, miR-29a and miR-448-3p in the Development of Cerebral Aneurysm."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Turk Neurosurg (2023)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.5137/1019-5149.JTN.41357-22.1"}], "href": "https://doi.org/10.5137/1019-5149.JTN.41357-22.1"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "36951025"}], "href": "https://pubmed.ncbi.nlm.nih.gov/36951025"}]}]}]}