{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nDiabetic nephropathy, a frequent complication of both type I and type II diabetes, is marked by the abnormal accumulation of extracellular matrix in the glomerular mesangium. In this context, previous proteomic studies have revealed that the proteasome subunit‐α type‐6 (PSMA6) protein is substantially down-regulated in nephropathic kidneys. This down-regulation was confirmed in diabetic patients using immunohistochemistry and further investigated in vitro using NRK-52E cells cultured under high glucose conditions."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "In the NRK-52E cell model, although the levels of PSMA6 mRNA remained largely unaltered under high glucose conditions, PSMA6 protein expression was significantly reduced. This discrepancy pointed to a post-transcriptional regulatory mechanism. Subsequent dual luciferase reporter assays established that miR-4490 binds directly to the PSMA6 mRNA, mediating its translational suppression."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "Furthermore, the inverse correlation observed between miR-4490 expression and PSMA6 protein levels, together with the modulation of PSMA6 expression upon exogenous adjustment of miR-4490, underscores the role of miR-4490 as a key regulator in diabetic nephropathy. These findings support the potential evaluation of miR-4490 as a biomarker for nephropathy in diabetic patients and provide a foundation for future therapeutic strategies targeting this microRNA."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Ying Feng, Ming-yue Jin, Dong-wei Liu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Proteasome subunit-α type-6 protein is post-transcriptionally repressed by the microRNA-4490 in diabetic nephropathy."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biosci Rep (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1042/BSR20180815"}], "href": "https://doi.org/10.1042/BSR20180815"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30287505"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30287505"}]}]}]}