{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nMicroRNA‐4649‑3p plays a tumor‐suppressive role in nasopharyngeal carcinoma (NPC) by directly targeting key regulatory molecules. In NPC cell lines, elevated levels of miR‑4649‑3p are associated with reduced expression of the tyrosine phosphatase SHP‑1. Functional experiments using miR‑4649‑3p mimics and inhibitors have demonstrated that up‐regulation of miR‑4649‑3p suppresses NPC cell proliferation through down‐regulation of SHP‑1, an effect that is reversible upon exogenous SHP‑1 expression. This inverse correlation underlines a direct regulatory relationship whereby miR‑4649‑3p limits tumor cell growth by targeting SHP‑1."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nIn a complementary study, in silico RNA crosstalk analyses revealed that miR‑4649‑3p can bind both to the long non‐coding RNA LINC02570 and to SREBF1 mRNA. In advanced-stage NPC, overexpression of LINC02570 appears to sequester miR‑4649‑3p, thereby diminishing its availability to suppress SREBF1. This molecular “sponge” effect leads to the up-regulation of SREBP1 and its downstream target, fatty acid synthase (FASN), promoting lipid metabolic changes that favor tumor progression."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Xiaofen Pan, Gang Peng, Sha Liu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "MicroRNA-4649-3p inhibits cell proliferation by targeting protein tyrosine phosphatase SHP-1 in nasopharyngeal carcinoma cells."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Int J Mol Med (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3892/ijmm.2015.2245"}], "href": "https://doi.org/10.3892/ijmm.2015.2245"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26081980"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26081980"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Fei Liu, Jiazhang Wei, Yanrong Hao, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Long intergenic non-protein coding RNA 02570 promotes nasopharyngeal carcinoma progression by adsorbing microRNA miR-4649-3p thereby upregulating both sterol regulatory element binding protein 1, and fatty acid synthase."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Bioengineered (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1080/21655979.2021.1979317"}], "href": "https://doi.org/10.1080/21655979.2021.1979317"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34546840"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34546840"}]}]}]}