{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nThe two studies provided focus on key regulators of branched‐chain amino acid (BCAA) metabolism and therapeutic interventions in disorders related to its dysregulation; however, neither abstract directly addresses or characterizes a function for MIR4705. Based on the available data, no explicit role or mechanistic insight regarding MIR4705 could be extracted from these reports."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nIn the first study, the disruption of the BDK gene in mice led to an uncontrolled oxidation of BCAAs due to constitutively active BCKDH complex activity. This deregulated metabolism resulted in multiple systemic effects including reduced BCAA levels, growth retardation, altered organ weights, and neurological impairment. Despite the detailed exploration of mechanisms governing BCKDH regulation and the importance of phosphorylation, the report did not mention or imply any function for MIR4705 in these processes."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nThe second study characterized a severe mouse model of maple syrup urine disease (MSUD) due to impaired activity of the mitochondrial branched‐chain 2-ketoacid dehydrogenase complex, and demonstrated that gene therapy targeting the BCKDHB gene can restore metabolic balance and rescue the phenotype. Although this study reinforces the therapeutic promise of targeting components of the BCKD complex in metabolic dysfunction, it does not provide any functional data or associations related to MIR4705."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Mandar A Joshi, Nam Ho Jeoung, Mariko Obayashi, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Impaired growth and neurological abnormalities in branched-chain alpha-keto acid dehydrogenase kinase-deficient mice."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biochem J (2006)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1042/BJ20060869"}], "href": "https://doi.org/10.1042/BJ20060869"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "16875466"}], "href": "https://pubmed.ncbi.nlm.nih.gov/16875466"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Clément Pontoizeau, Clovis Gaborit, Nolan Tual, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Successful treatment of severe MSUD in Bckdhb"}, {"type": "a", "children": [{"type": "t", "text": "sup"}], "href": "sup"}, {"type": "t", "text": "-/-"}, {"type": "a", "children": [{"type": "t", "text": "/sup"}], "href": "/sup"}, {"type": "t", "text": " mice with neonatal AAV gene therapy."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Inherit Metab Dis (2024)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1002/jimd.12604"}], "href": "https://doi.org/10.1002/jimd.12604"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "36880392"}], "href": "https://pubmed.ncbi.nlm.nih.gov/36880392"}]}]}]}