{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nRecent studies have revealed that microRNA‐4712‐5p plays a critical oncogenic role in vulvar squamous cell carcinoma (VSCC). Its expression is significantly increased in VSCC tissues and in the A431 cell line, and this upregulation is associated with enhanced cell proliferation, invasion, and metastasis. Mechanistically, microRNA‐4712‐5p directly targets the phosphatase and tensin homologue (PTEN) gene, leading to its marked downregulation. This decrease in PTEN levels disrupts its normal inhibitory influence on downstream signaling pathways, evidenced by a significant increase in phosphorylated AKT (p‐AKT) and cyclin D1, along with a concomitant decrease in phosphorylated GSK3β, collectively fostering an environment that supports tumor progression."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nFunctional assays, including reverse transcription‐quantitative polymerase chain reaction, Dual-Luciferase® Reporter Assay, and western blot analysis, have confirmed that overexpression of microRNA‐4712‐5p in VSCC not only reduces PTEN expression but also modulates its downstream effectors. These molecular alterations contribute to the aggressive behavior observed in VSCC, underscoring the potential of microRNA‐4712‐5p as both a biomarker and a therapeutic target in the management of this malignancy."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Shaojie Yang, Yanyan Zhao, Lufang Wang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "MicroRNA‑4712‑5p promotes proliferation of the vulvar squamous cell carcinoma cell line A431 by targeting PTEN through the AKT/cyclin D1 signaling pathways."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Oncol Rep (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3892/or.2019.7320"}], "href": "https://doi.org/10.3892/or.2019.7320"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "31545465"}], "href": "https://pubmed.ncbi.nlm.nih.gov/31545465"}]}]}]}