{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nRecent studies have demonstrated that CLEC12B is a C‐type lectin receptor with a highly specific expression pattern in skin mast cells. Using a novel monoclonal antibody, researchers found that CLEC12B is exclusively expressed on mast cells in the skin of both mice and humans, suggesting that it plays a specialized role in regulating mast cell activation and cutaneous immune responses. This restricted expression pattern implicates CLEC12B as an important regulator in allergic responses and host defense mechanisms within the skin."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nAdditional research has extended the functional profile of CLEC12B by uncovering its role as a pattern recognition receptor in innate immunity. Binding studies revealed that CLEC12B recognizes specific carbohydrate moieties—including β-(1,3)-glucans and N-acetylglucosamine residues—present in the cell walls of Pneumocystis species. Functional analyses using siRNA-mediated silencing showed that inhibition of CLEC12B leads to a significant reduction in TNF-α production by macrophages in response to Pneumocystis stimuli, thereby highlighting its contributory role in the pro-inflammatory signaling cascade during infection."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nCollectively, the findings from these studies indicate that CLEC12B functions as an immunomodulatory receptor with dual roles: it regulates mast cell activation in the skin and mediates the innate inflammatory response to fungal pathogens. Although the query mentioned MIR4716, the abstracts consistently focus on CLEC12B, revealing its importance in both cutaneous immune homeostasis and pathogen recognition. Further research employing CLEC12B-deficient models will be essential to fully delineate the receptor’s signaling pathways and its potential as a therapeutic target in immune-mediated disorders."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Ayana Iijima, Kazumasa Kanemaru, Yaqiu Wang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Selective expression of a C-type lectin receptor, Clec12b, on skin mast cells."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biochem Biophys Res Commun (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.bbrc.2021.04.097"}], "href": "https://doi.org/10.1016/j.bbrc.2021.04.097"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34020140"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34020140"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Theodore J Kottom, Eva M Carmona, Kyle Schaefbauer, et al. "}, {"type": "b", "children": [{"type": "t", "text": "CLEC4A and CLEC12B C-type lectin receptors mediate interactions with "}, {"type": "a", "children": [{"type": "t", "text": "i"}], "href": "i"}, {"type": "t", "text": "Pneumocystis"}, {"type": "a", "children": [{"type": "t", "text": "/i"}], "href": "/i"}, {"type": "t", "text": " cell wall components."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Med Microbiol (2023)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1099/jmm.0.001714"}], "href": "https://doi.org/10.1099/jmm.0.001714"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "37294293"}], "href": "https://pubmed.ncbi.nlm.nih.gov/37294293"}]}]}]}