{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nRecent studies have uncovered that miR-4733-5p is significantly upregulated in gallbladder carcinoma (GBC), a highly aggressive cancer associated with poor prognosis, and that this miRNA plays a critical role in promoting the proliferation and invasion of GBC cells. The increased expression of miR-4733-5p was validated using GEO dataset analyses, RT-qPCR, ISH staining, and immunohistochemistry, which collectively indicate its involvement in tumor progression by enhancing cell growth and metastasis."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "Mechanistically, miR-4733-5p exerts its oncogenic effects by directly binding to the 3′ untranslated region (UTR) of the mRNA encoding the tumor suppressor kruppel‐like factor 7 (KLF7). This interaction leads to the downregulation of KLF7, which normally acts to inhibit GBC cell proliferation and migration; thus, loss of KLF7 function removes a critical brake on tumor development."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "A comprehensive suite of in vitro functional assays—including CCK-8, colony formation, wound healing, and transwell invasion assays—along with xenograft tumor models in vivo, substantiated the role of miR-4733-5p in advancing GBC tumor growth. These findings suggest that targeting miR-4733-5p may provide therapeutic value for managing gallbladder carcinoma by restoring the expression and function of KLF7."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Xiaoqiang Hu, Junzhe Zhang, Junfeng Bu, et al. "}, {"type": "b", "children": [{"type": "t", "text": "MiR-4733-5p promotes gallbladder carcinoma progression via directly targeting kruppel like factor 7."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Bioengineered (2022)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1080/21655979.2022.2065951"}], "href": "https://doi.org/10.1080/21655979.2022.2065951"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "35443866"}], "href": "https://pubmed.ncbi.nlm.nih.gov/35443866"}]}]}]}