{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nThe study reported in the abstract"}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "describes the identification and characterization of a novel cyclic nucleotide-binding domain (CNBD)‐containing protein, designated CRIS, which is exclusively expressed in sperm precursor cells. CRIS binds the second messengers cAMP and cGMP via its conserved CNBD and plays a crucial role during spermiogenesis. Loss of CRIS in male mice leads either to a complete spermatogenic arrest with resultant infertility or to impaired sperm motility, which is associated with abnormal Ca²⁺ regulation of flagellar beat asymmetry that mimics sperm hyperactivation. Furthermore, the results indicate that CRIS interacts with Ca²⁺‐regulated proteins during the formation of sperm, which are later important for the bending of the flagellum during mature sperm function. It is important to note that although the user’s query asked about MIR4745, the provided abstract does not contain information regarding MIR4745 and instead focuses exclusively on the role of CRIS in regulating sperm function and fertility.\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Anke Miriam Krähling, Luis Alvarez, Katharina Debowski, et al. "}, {"type": "b", "children": [{"type": "t", "text": "CRIS-a novel cAMP-binding protein controlling spermiogenesis and the development of flagellar bending."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS Genet (2013)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pgen.1003960"}], "href": "https://doi.org/10.1371/journal.pgen.1003960"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "24339785"}], "href": "https://pubmed.ncbi.nlm.nih.gov/24339785"}]}]}]}