{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nIn the context of influenza A virus (IAV) infection, miR-4776-1 plays a crucial regulatory role by targeting the mRNA of NFKBIB, an inhibitor that normally restrains NF-κB activity. Early in infection, the expression of miR-4776-1 is increased, leading to a reduction in NFKBIB levels and thereby potentially enhancing NF-κB signaling. This early reduction in NFKBIB is followed by a downregulation of miR-4776-1, which allows NFKBIB expression to recover, suggesting a dynamic, time-dependent modulation of the NF-κB pathway. Functional assays, including miRNA pull-down and 3′ UTR luciferase reporter experiments, confirmed that miR-4776-1 directly binds to NFKBIB mRNA. Importantly, overexpression of NFKBIB in IAV-infected cells led to decreased viral levels, indicating that miR-4776-1 indirectly influences IAV production by modulating the NF-κB signaling cascade through its regulation of NFKBIB."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Sreekumar Othumpangat, Nicole B Bryan, Donald H Beezhold, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Upregulation of miRNA-4776 in Influenza Virus Infected Bronchial Epithelial Cells Is Associated with Downregulation of NFKBIB and Increased Viral Survival."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Viruses (2017)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3390/v9050094"}], "href": "https://doi.org/10.3390/v9050094"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "28448456"}], "href": "https://pubmed.ncbi.nlm.nih.gov/28448456"}]}]}]}