{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nMIR4776-2 plays a regulatory role during Influenza A virus (IAV) infection by directly targeting the messenger RNA of NF-κB inhibitor beta (NFKBIB), a protein known to sequester NF-κB in the cytoplasm. Upon IAV exposure, MIR4776-2 is rapidly upregulated within the first hour, leading to a decrease in NFKBIB expression, which in turn may favor the activation of NF-κB signaling. This modulation is time-dependent, as MIR4776-2 levels later diminish around four hours post-infection, coinciding with a rebound in NFKBIB expression. Functional studies, including miRNA ago-immunoprecipitation and a 3′ UTR luciferase assay, have confirmed that MIR4776-2 directly interacts with NFKBIB mRNA, and transfection of a MIR4776-2 mimic in uninfected cells similarly led to reduced levels of NFKBIB. These findings suggest that by finely tuning NFKBIB expression, MIR4776-2 indirectly modulates NF-κB activity, thereby influencing IAV production and potentially contributing to the host’s early antiviral response."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Sreekumar Othumpangat, Nicole B Bryan, Donald H Beezhold, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Upregulation of miRNA-4776 in Influenza Virus Infected Bronchial Epithelial Cells Is Associated with Downregulation of NFKBIB and Increased Viral Survival."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Viruses (2017)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3390/v9050094"}], "href": "https://doi.org/10.3390/v9050094"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "28448456"}], "href": "https://pubmed.ncbi.nlm.nih.gov/28448456"}]}]}]}