MIR514A1 Gene

HGNC Family Non-coding RNAs
Name microRNA 514a-1
Description microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop. [provided by RefSeq, Sep 2009]
Summary
{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nMicroRNA‐514a-3p (encoded by MIR514A1) has been identified as a melanoma-specific miRNA that is markedly overexpressed in melanoma compared to other solid cancers. It forms part of the miR-506–514 cluster and has been implicated in the initiation of melanocyte transformation and promotion of melanoma growth. Functional studies reveal that miR‐514a-3p modulates the expression of numerous genes—including CTNNB1, CDK2, MC1R, and notably NF1—thereby contributing to the regulation of key signaling pathways. Direct targeting of NF1 by miR‐514a-3p, as confirmed by luciferase-reporter assays, results in decreased NF1 levels; this downregulation is associated with enhanced survival of BRAFV600E melanoma cells treated with targeted inhibitors, providing a mechanistic insight into drug resistance in melanoma."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nIn addition to its role in melanoma, miR‐514a-3p exerts significant function in testicular germ cell tumors. In these tumors, overexpression of miR‐514a-3p induces apoptosis through the direct suppression of the paternally expressed gene 3 (PEG3). Loss or downregulation of miR‐514a-3p leads to increased PEG3 expression, which in turn recruits TRAF2 and activates the NF‑κB pathway, ultimately protecting tumor cells from apoptosis. These findings underscore a context-dependent role for MIR514A1, where its regulatory impact on PEG3 and NF‑κB signaling balances cell survival and death, thereby contributing to tumor progression."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Mitchell S Stark, Vanessa F Bonazzi, Glen M Boyle, et al. "}, {"type": "b", "children": [{"type": "t", "text": "miR-514a regulates the tumour suppressor NF1 and modulates BRAFi sensitivity in melanoma."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Oncotarget (2015)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.18632/oncotarget.3924"}], "href": "https://doi.org/10.18632/oncotarget.3924"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "25980496"}], "href": "https://pubmed.ncbi.nlm.nih.gov/25980496"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Deniz Mahmut Özata, Xidan Li, Linkiat Lee, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Loss of miR-514a-3p regulation of PEG3 activates the NF-kappa B pathway in human testicular germ cell tumors."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cell Death Dis (2017)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/cddis.2016.464"}], "href": "https://doi.org/10.1038/cddis.2016.464"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "28471449"}], "href": "https://pubmed.ncbi.nlm.nih.gov/28471449"}]}]}]}
Synonyms MIRN514-1, MIR-514A-1, HSA-MIR-514A-1, MIR514-1
NCBI Gene ID 574516
API
Download Associations
Predicted Functions View MIR514A1's ARCHS4 Predicted Functions.
Co-expressed Genes View MIR514A1's ARCHS4 Predicted Functions.
Expression in Tissues and Cell Lines View MIR514A1's ARCHS4 Predicted Functions.

Functional Associations

MIR514A1 has 157 functional associations with biological entities spanning 4 categories (molecular profile, functional term, phrase or reference, cell line, cell type or tissue, gene, protein or microRNA) extracted from 8 datasets.

Click the + buttons to view associations for MIR514A1 from the datasets below.

If available, associations are ranked by standardized value

Dataset Summary
CCLE Cell Line Gene CNV Profiles cell lines with high or low copy number of MIR514A1 gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
COSMIC Cell Line Gene CNV Profiles cell lines with high or low copy number of MIR514A1 gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset.
ENCODE Transcription Factor Binding Site Profiles transcription factor binding site profiles with transcription factor binding evidence at the promoter of MIR514A1 gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
ENCODE Transcription Factor Targets transcription factors binding the promoter of MIR514A1 gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.
GeneSigDB Published Gene Signatures PubMedIDs of publications reporting gene signatures containing MIR514A1 from the GeneSigDB Published Gene Signatures dataset.
JASPAR Predicted Transcription Factor Targets transcription factors regulating expression of MIR514A1 gene predicted using known transcription factor binding site motifs from the JASPAR Predicted Transcription Factor Targets dataset.
Klijn et al., Nat. Biotechnol., 2015 Cell Line Gene CNV Profiles cell lines with high or low copy number of MIR514A1 gene relative to other cell lines from the Klijn et al., Nat. Biotechnol., 2015 Cell Line Gene CNV Profiles dataset.
MotifMap Predicted Transcription Factor Targets transcription factors regulating expression of MIR514A1 gene predicted using known transcription factor binding site motifs from the MotifMap Predicted Transcription Factor Targets dataset.