MIR516B1 Gene

HGNC Family Non-coding RNAs
Name microRNA 516b-1
Description microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop. [provided by RefSeq, Sep 2009]
Summary
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In non‑small cell lung cancer (NSCLC), the lncRNA FEZF1‑AS1 is upregulated and acts as an oncogene by sponging miR‑516b‑5p, which relieves the microRNA’s suppression on ITGA11 and thereby promotes cell proliferation and migration."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": " A similar mechanism occurs in osteosarcoma, where LINC01123 binds miR‑516b‑5p to enable increased expression of the transcriptional activator Gli1, contributing to proliferation and metastasis."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": " In melanoma, the circular RNA circ_0079593 acts as a sponge for miR‑516b, resulting in the derepression of GRM3, which in turn accelerates tumor cell proliferation, migration, invasion, and glucose metabolism."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": " Furthermore, in hepatocellular carcinoma, the lncRNA LINC02362 sequesters miR‑516b‑5p, leading to upregulation of SOSC2 and suppression of tumor‑suppressive pathways."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "5"}]}, {"type": "t", "text": " In thyroid cancer, ARAP1‑AS1 similarly contributes to tumor development by sponging miR‑516b‑5p to modulate PDE5A expression."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "6"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nMoreover, direct functional studies in glioblastoma have demonstrated that overexpressing miR‑516b‑5p can significantly impair cell migration and invasion, underscoring its key role in controlling metastatic behavior."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "7"}]}, {"type": "t", "text": " Collectively, these findings indicate that miR‑516b‑5p acts as a central tumor suppressor in multiple cancer models, where its inhibition—often via sponging by various non‑coding RNAs—leads to the de‑repression of oncogenic targets. This multifaceted regulatory network positions miR‑516b‑5p as a promising biomarker and potential therapeutic target in cancer.\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Yufeng Zhao, Yaqiang Wang, Guochen Xing "}, {"type": "b", "children": [{"type": "t", "text": "miR-516b functions as a tumor suppressor by directly modulating CCNG1 expression in esophageal squamous cell carcinoma."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biomed Pharmacother (2018)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.biopha.2018.07.074"}], "href": "https://doi.org/10.1016/j.biopha.2018.07.074"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "30119241"}], "href": "https://pubmed.ncbi.nlm.nih.gov/30119241"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Heng Song, Hui Li, Xiaosong Ding, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Long non‑coding RNA FEZF1‑AS1 facilitates non‑small cell lung cancer progression via the ITGA11/miR‑516b‑5p axis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Int J Oncol (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.3892/ijo.2020.5142"}], "href": "https://doi.org/10.3892/ijo.2020.5142"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "33174014"}], "href": "https://pubmed.ncbi.nlm.nih.gov/33174014"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Xiaohui Pan, Jingxue Tan, Tao Tao, et al. "}, {"type": "b", "children": [{"type": "t", "text": "LINC01123 enhances osteosarcoma cell growth by activating the Hedgehog pathway via the miR-516b-5p/Gli1 axis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cancer Sci (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1111/cas.14913"}], "href": "https://doi.org/10.1111/cas.14913"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "33837611"}], "href": "https://pubmed.ncbi.nlm.nih.gov/33837611"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Jiajing Lu, Ying Li "}, {"type": "b", "children": [{"type": "t", "text": "Circ_0079593 facilitates proliferation, metastasis, glucose metabolism and inhibits apoptosis in melanoma by regulating the miR-516b/GRM3 axis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Cell Biochem (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1007/s11010-020-03875-8"}], "href": "https://doi.org/10.1007/s11010-020-03875-8"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "32839935"}], "href": "https://pubmed.ncbi.nlm.nih.gov/32839935"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Dezhi Li, Tao Zhou, Yaqin Li, et al. "}, {"type": "b", "children": [{"type": "a", "children": [{"type": "t", "text": "i"}], "href": "i"}, {"type": "t", "text": "LINC02362"}, {"type": "a", "children": [{"type": "t", "text": "/i"}], "href": "/i"}, {"type": "t", "text": " attenuates hepatocellular carcinoma progression through the "}, {"type": "a", "children": [{"type": "t", "text": "i"}], "href": "i"}, {"type": "t", "text": "miR-516b-5p"}, {"type": "a", "children": [{"type": "t", "text": "/i"}], "href": "/i"}, {"type": "t", "text": "/"}, {"type": "a", "children": [{"type": "t", "text": "i"}], "href": "i"}, {"type": "t", "text": "SOSC2"}, {"type": "a", "children": [{"type": "t", "text": "/i"}], "href": "/i"}, {"type": "t", "text": " axis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Aging (Albany NY) (2022)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.18632/aging.203813"}], "href": "https://doi.org/10.18632/aging.203813"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34990401"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34990401"}]}, {"type": "r", "ref": 6, "children": [{"type": "t", "text": "Qiuli Du, Lin Huang, Wei Guo "}, {"type": "b", "children": [{"type": "t", "text": "LncRNA ARAP1-AS1 targets miR-516b-5p/PDE5A axis to facilitate the progression of thyroid cancer."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Anticancer Drugs (2023)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1097/CAD.0000000000001438"}], "href": "https://doi.org/10.1097/CAD.0000000000001438"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "36730555"}], "href": "https://pubmed.ncbi.nlm.nih.gov/36730555"}]}, {"type": "r", "ref": 7, "children": [{"type": "t", "text": "Ángela Y García Fonseca, Yeimy González-Giraldo, Jannet Gonzalez Santos, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The hsa-miR-516a-5p and hsa-miR-516b-5p microRNAs reduce the migration and invasion on T98G glioblastoma cell line."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cancer Genet (2023)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.cancergen.2022.11.002"}], "href": "https://doi.org/10.1016/j.cancergen.2022.11.002"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "36410106"}], "href": "https://pubmed.ncbi.nlm.nih.gov/36410106"}]}]}]}
Synonyms MIR-516B-1, MIRN516-4, MIRN516B-1, MIRN516B1
NCBI Gene ID 574490
API
Download Associations
Predicted Functions View MIR516B1's ARCHS4 Predicted Functions.
Co-expressed Genes View MIR516B1's ARCHS4 Predicted Functions.
Expression in Tissues and Cell Lines View MIR516B1's ARCHS4 Predicted Functions.

Functional Associations

MIR516B1 has 178 functional associations with biological entities spanning 4 categories (molecular profile, chemical, cell line, cell type or tissue, gene, protein or microRNA) extracted from 10 datasets.

Click the + buttons to view associations for MIR516B1 from the datasets below.

If available, associations are ranked by standardized value

Dataset Summary
CCLE Cell Line Gene CNV Profiles cell lines with high or low copy number of MIR516B1 gene relative to other cell lines from the CCLE Cell Line Gene CNV Profiles dataset.
COSMIC Cell Line Gene CNV Profiles cell lines with high or low copy number of MIR516B1 gene relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset.
ENCODE Histone Modification Site Profiles histone modification site profiles with high histone modification abundance at MIR516B1 gene from the ENCODE Histone Modification Site Profiles dataset.
ENCODE Transcription Factor Binding Site Profiles transcription factor binding site profiles with transcription factor binding evidence at the promoter of MIR516B1 gene from the ENCODE Transcription Factor Binding Site Profiles dataset.
ENCODE Transcription Factor Targets transcription factors binding the promoter of MIR516B1 gene in ChIP-seq datasets from the ENCODE Transcription Factor Targets dataset.
GEO Signatures of Differentially Expressed Genes for Kinase Perturbations kinase perturbations changing expression of MIR516B1 gene from the GEO Signatures of Differentially Expressed Genes for Kinase Perturbations dataset.
GEO Signatures of Differentially Expressed Genes for Small Molecules small molecule perturbations changing expression of MIR516B1 gene from the GEO Signatures of Differentially Expressed Genes for Small Molecules dataset.
Klijn et al., Nat. Biotechnol., 2015 Cell Line Gene CNV Profiles cell lines with high or low copy number of MIR516B1 gene relative to other cell lines from the Klijn et al., Nat. Biotechnol., 2015 Cell Line Gene CNV Profiles dataset.
KnockTF Gene Expression Profiles with Transcription Factor Perturbations transcription factor perturbations changing expression of MIR516B1 gene from the KnockTF Gene Expression Profiles with Transcription Factor Perturbations dataset.
Roadmap Epigenomics Histone Modification Site Profiles histone modification site profiles with high histone modification abundance at MIR516B1 gene from the Roadmap Epigenomics Histone Modification Site Profiles dataset.