{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nIn basal‐like breast cancer, microRNA‐5694 (miR-5694) plays a critical role in promoting metastasis by directly targeting and degrading the mRNA of the metastasis suppressor gene AF9. Loss of AF9 disrupts its normal function of repressing the epithelial-mesenchymal transition (EMT) regulator Snail, which normally acts to restrain EMT. The reduction of AF9 levels by miR-5694 thus results in increased cellular invasion and migration, conferring a metastatic advantage to the cancer cells."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nMechanistic investigations revealed that AF9 normally interacts with Snail, hampering its transcriptional activity and maintaining an active chromatin state on the promoters of genes involved in EMT via recruitment of histone acetyltransferases like CBP and GCN5. When miR-5694 mediates the degradation of AF9 mRNA, this checkpoint is removed, leading to an unchecked activation of Snail and subsequently enhanced EMT features that contribute to the metastatic behavior of the tumor."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nThese findings underscore the potential of targeting the miR-5694/AF9 axis as a therapeutic strategy in managing metastatic breast cancer. Restoring AF9 levels could counteract the pro-metastatic effects mediated by miR-5694, thereby impeding EMT and tumor dissemination."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Xin Tian, Hua Yu, Dong Li, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The miR-5694/AF9/Snail Axis Provides Metastatic Advantages and a Therapeutic Target in Basal-like Breast Cancer."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Ther (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.ymthe.2020.11.022"}], "href": "https://doi.org/10.1016/j.ymthe.2020.11.022"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "33221433"}], "href": "https://pubmed.ncbi.nlm.nih.gov/33221433"}]}]}]}