{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nRecent high‐throughput serum miRNA screenings in patients with chronic liver diseases and type 2 diabetes mellitus (T2DM) have identified miR-571 as one of several miRNAs whose levels are significantly altered. In a study of chronic liver disease and cirrhosis, a systematic screening approach was undertaken to find serum miRNAs with differential expression, suggesting that such molecules, including miR-571, could play a role in modulating disease pathology."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": " Further, serum analyses in T2DM patients, particularly those with microvascular complications and diabetic foot ulcers, revealed that miR-571 is markedly upregulated and correlated with the progression of these complications, indicating its potential utility as a biomarker and risk factor in diabetes-associated vascular injury."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nExperimental evidence has also uncovered a direct functional role for miR-571 in the maintenance of genomic stability. In cancer cells, miR-571 was found to target Geminin—a critical inhibitor of DNA re-replication—thereby reducing Geminin protein levels during late S-phase independently of the anaphase-promoting complex. This regulation of DNA replication and S-phase progression is orchestrated by a Cdk2-c-Myc-miR-571 axis, highlighting its importance in controlling DNA replication and preserving genomic integrity."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "4"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nIn addition to its roles in diabetic complications and genomic stability, miR-571 appears to participate in the progression of liver fibrosis. Elevated in patients with liver fibrosis, miR-571 promotes the activation of human hepatic stellate cells by enhancing proliferation, migration, and inhibiting apoptosis. Mechanistically, increased miR-571 levels upregulate components of the Notch3 signaling pathway—specifically Notch3 and Jagged1—which in turn stimulates fibrogenic processes. These findings suggest that miR-571 not only serves as a potential marker of fibrotic progression but also as a mediator of hepatic stellate cell activation and liver tissue remodeling."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "5"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Christoph Roderburg, Tobias Mollnow, Brenda Bongaerts, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Micro-RNA profiling in human serum reveals compartment-specific roles of miR-571 and miR-652 in liver cirrhosis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "PLoS One (2012)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1371/journal.pone.0032999"}], "href": "https://doi.org/10.1371/journal.pone.0032999"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "22412969"}], "href": "https://pubmed.ncbi.nlm.nih.gov/22412969"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Cheng Wang, Shujun Wan, Ting Yang, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Increased serum microRNAs are closely associated with the presence of microvascular complications in type 2 diabetes mellitus."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Sci Rep (2016)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/srep20032"}], "href": "https://doi.org/10.1038/srep20032"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "26831044"}], "href": "https://pubmed.ncbi.nlm.nih.gov/26831044"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Rashin VatanIman, Seyed Hossein Malekpour, Afsoon Afshari, et al. "}, {"type": "b", "children": [{"type": "t", "text": "MiR-770-5p, miR-661 and miR-571 expression level in serum and tissue samples of foot ulcer caused by diabetes mellitus type II in Iranian population."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Mol Biol Rep (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1007/s11033-021-06798-9"}], "href": "https://doi.org/10.1007/s11033-021-06798-9"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34643918"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34643918"}]}, {"type": "r", "ref": 4, "children": [{"type": "t", "text": "Yi Zhang, Zhuqing Li, Qiang Hao, et al. "}, {"type": "b", "children": [{"type": "t", "text": "The Cdk2-c-Myc-miR-571 Axis Regulates DNA Replication and Genomic Stability by Targeting Geminin."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Cancer Res (2019)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1158/0008-5472.CAN-19-0020"}], "href": "https://doi.org/10.1158/0008-5472.CAN-19-0020"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "31431461"}], "href": "https://pubmed.ncbi.nlm.nih.gov/31431461"}]}, {"type": "r", "ref": 5, "children": [{"type": "t", "text": "Shuo Cong, Yongmei Liu, Yi Li, et al. "}, {"type": "b", "children": [{"type": "t", "text": "MiR-571 affects the development and progression of liver fibrosis by regulating the Notch3 pathway."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Sci Rep (2021)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1038/s41598-021-00638-3"}], "href": "https://doi.org/10.1038/s41598-021-00638-3"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "34750395"}], "href": "https://pubmed.ncbi.nlm.nih.gov/34750395"}]}]}]}