{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nThe study demonstrates that miR-6071 acts as a tumor suppressor in hepatocellular carcinoma by regulating liver cancer stem cells (LCSCs). Reduced miR-6071 levels were observed in LCSCs, and its overexpression led to decreased self-renewal, proliferation, migration, and tumorigenic capabilities of HCC cells. Mechanistically, miR-6071 directly targets the 3′UTR of PTPN11 mRNA, resulting in downregulation of this oncogenic phosphatase, as confirmed by bioinformatics analysis and luciferase reporter assays. A significant negative correlation between miR-6071 expression and PTPN11 levels was found in clinical HCC samples, supporting the regulatory link between the two. Functionally, interference with PTPN11, as well as the use of the inhibitor IACS-13909, nullified the effects of miR-6071 modulation on LCSC properties, underscoring the importance of the miR-6071–PTPN11 axis. Furthermore, miR-6071 upregulation enhanced the sensitivity of HCC cells to lenvatinib treatment, and clinical data suggested that higher miR-6071 expression is associated with improved postoperative treatment outcomes."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": ""}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Minyong Chen, Huaxiang Wang, Songchang Shi, et al. "}, {"type": "b", "children": [{"type": "t", "text": "miR-6071 inhibits hepatocellular carcinoma progression via targeting PTPN11."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Arch Biochem Biophys (2022)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1016/j.abb.2022.109345"}], "href": "https://doi.org/10.1016/j.abb.2022.109345"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "35792156"}], "href": "https://pubmed.ncbi.nlm.nih.gov/35792156"}]}]}]}