{"type": "root", "children": [{"type": "p", "children": [{"type": "t", "text": "\nHsa‐miR-6165 is a microRNA located within the fourth intron of the p75NTR gene and is expressed from an independent promoter that can be epigenetically modulated. In human cell lines such as HCT116, HeLa, NT2, SW480, and HEK293T, overexpression of miR-6165 leads to the downregulation of several target genes—including ABLIM-1, PVRL1, PKD1, PLXNA2, and PDK1—which in turn affects neural differentiation, cell cycle progression, and may increase apoptosis. The independent regulation of miR-6165, distinct from its host gene, suggests that it contributes additional complexity to p75NTR-associated cellular functions."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "1"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nIn colorectal cancer models, miR-6165 has been shown to exert tumor suppressive effects. Overexpression of miR-6165 in SW480 cells leads to a direct interaction with the 3′UTR of the insulin-like growth factor 1 receptor (IGF-1R) gene, resulting in substantial downregulation of IGF-1R and downstream oncogenic signals—including AKT2, AKT3, PI3KR3, PI3KR5, CCND1, c-MYC, and P21 transcripts—which ultimately promotes apoptosis and reduces cellular viability. Moreover, an inverse expression pattern of miR-6165 and IGF-1R during NT2 cell neural differentiation strengthens the concept of its key regulatory role in both developmental and oncogenic contexts."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "2"}]}, {"type": "t", "text": "\n"}]}, {"type": "t", "text": "\n\n"}, {"type": "p", "children": [{"type": "t", "text": "\nAdditionally, the functional landscape of miR-6165 is further enriched by its interaction with noncoding RNAs in hepatocellular carcinoma. The circular RNA circ_0001459 has been identified as a molecular sponge for miR-6165, thereby mitigating its inhibitory effect on IGF1R expression. This sponging action contributes to the upregulation of IGF1R, leading to enhanced epithelial-to-mesenchymal transition and promoting HCC progression and metastasis. Thus, the interplay between circ_0001459 and miR-6165 underscores a novel regulatory axis in liver cancer."}, {"type": "fg", "children": [{"type": "fg_f", "ref": "3"}]}, {"type": "t", "text": "\n"}]}, {"type": "rg", "children": [{"type": "r", "ref": 1, "children": [{"type": "t", "text": "Maryam Hassanlou, Bahram Mohammad Soltani, Seyed Javad Mowla "}, {"type": "b", "children": [{"type": "t", "text": "Expression and Function of hsa-miR-6165 in Human Cell Lines and During the NT2 Cell Neural Differentiation Process."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "J Mol Neurosci (2017)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1007/s12031-017-0954-5"}], "href": "https://doi.org/10.1007/s12031-017-0954-5"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "28956260"}], "href": "https://pubmed.ncbi.nlm.nih.gov/28956260"}]}, {"type": "r", "ref": 2, "children": [{"type": "t", "text": "Maryam Hassanlou, Bahram M Soltani, Abdallah Medlej, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Hsa-miR-6165 downregulates insulin-like growth factor-1 receptor (IGF-1R) expression and enhances apoptosis in SW480 cells."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Biol Chem (2020)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1515/hsz-2018-0421"}], "href": "https://doi.org/10.1515/hsz-2018-0421"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "31702994"}], "href": "https://pubmed.ncbi.nlm.nih.gov/31702994"}]}, {"type": "r", "ref": 3, "children": [{"type": "t", "text": "Duo Shen, Hongyu Zhao, Peng Zeng, et al. "}, {"type": "b", "children": [{"type": "t", "text": "Circular RNA circ_0001459 accelerates hepatocellular carcinoma progression via the miR-6165/IGF1R axis."}]}, {"type": "t", "text": " "}, {"type": "i", "children": [{"type": "t", "text": "Ann N Y Acad Sci (2022)"}]}, {"type": "t", "text": " DOI: "}, {"type": "a", "children": [{"type": "t", "text": "10.1111/nyas.14753"}], "href": "https://doi.org/10.1111/nyas.14753"}, {"type": "t", "text": " PMID: "}, {"type": "a", "children": [{"type": "t", "text": "35199365"}], "href": "https://pubmed.ncbi.nlm.nih.gov/35199365"}]}]}]}